Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1532169..1532823 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag MJ614_RS09610 Protein ID WP_000244772.1
Coordinates 1532169..1532576 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MJ614_RS09615 Protein ID WP_000354046.1
Coordinates 1532557..1532823 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS09590 (1528126) 1528126..1529859 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
MJ614_RS09595 (1529865) 1529865..1530575 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MJ614_RS09600 (1530600) 1530600..1531496 - 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
MJ614_RS09605 (1531608) 1531608..1532129 + 522 WP_001055874.1 flavodoxin FldB -
MJ614_RS09610 (1532169) 1532169..1532576 - 408 WP_000244772.1 protein YgfX Toxin
MJ614_RS09615 (1532557) 1532557..1532823 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MJ614_RS09620 (1533066) 1533066..1534046 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
MJ614_RS09625 (1534242) 1534242..1534901 - 660 WP_000250274.1 hemolysin III family protein -
MJ614_RS09630 (1535065) 1535065..1535376 - 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
MJ614_RS09635 (1535421) 1535421..1536854 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
MJ614_RS09640 (1536911) 1536911..1537654 - 744 WP_000951941.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T236858 WP_000244772.1 NZ_CP092647:c1532576-1532169 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T236858 NZ_LR740776:c1554188-1553910 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT236858 WP_000354046.1 NZ_CP092647:c1532823-1532557 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236858 NZ_LR740776:c1553910-1553626 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References