Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1389389..1389972 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag MJ614_RS08955 Protein ID WP_000254745.1
Coordinates 1389389..1389724 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MJ614_RS08960 Protein ID WP_000581937.1
Coordinates 1389724..1389972 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS08940 (1385276) 1385276..1386574 - 1299 WP_000036723.1 phosphopyruvate hydratase -
MJ614_RS08945 (1386662) 1386662..1388299 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
MJ614_RS08950 (1388527) 1388527..1389318 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
MJ614_RS08955 (1389389) 1389389..1389724 - 336 WP_000254745.1 endoribonuclease MazF Toxin
MJ614_RS08960 (1389724) 1389724..1389972 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MJ614_RS08965 (1390050) 1390050..1392284 - 2235 WP_000226815.1 GTP pyrophosphokinase -
MJ614_RS08970 (1392332) 1392332..1393633 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T236857 WP_000254745.1 NZ_CP092647:c1389724-1389389 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T236857 NZ_LR740776:c1270528-1270421 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT236857 WP_000581937.1 NZ_CP092647:c1389972-1389724 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT236857 NZ_LR740776:1270581-1270642 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References