Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1061614..1062239 Replicon chromosome
Accession NZ_CP092647
Organism Escherichia coli strain EC5502

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag MJ614_RS07295 Protein ID WP_000911329.1
Coordinates 1061614..1062012 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag MJ614_RS07300 Protein ID WP_000450524.1
Coordinates 1062012..1062239 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ614_RS07275 (1057492) 1057492..1057692 + 201 WP_000383836.1 YpfN family protein -
MJ614_RS07280 (1057802) 1057802..1058500 - 699 WP_000679823.1 esterase -
MJ614_RS07285 (1058574) 1058574..1060589 - 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
MJ614_RS07290 (1060604) 1060604..1061467 - 864 WP_001267498.1 neutral zinc metallopeptidase -
MJ614_RS07295 (1061614) 1061614..1062012 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
MJ614_RS07300 (1062012) 1062012..1062239 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
MJ614_RS07305 (1062394) 1062394..1063107 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
MJ614_RS07310 (1063320) 1063320..1064354 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
MJ614_RS07315 (1064371) 1064371..1065249 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
MJ614_RS07320 (1065395) 1065395..1065967 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
MJ614_RS07325 (1065967) 1065967..1066437 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T236856 WP_000911329.1 NZ_CP092647:c1062012-1061614 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T236856 NZ_LR740776:543579-543881 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT236856 WP_000450524.1 NZ_CP092647:c1062239-1062012 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT236856 NZ_LR740776:543917-544258 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References