Detailed information of TA system TA236846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 20423..22046
• Replicon: plasmid pEC5502-NDM5
• Accession: NZ_CP092646
• Organism: Escherichia coli strain EC5502

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: MJ614_RS00680
• Protein ID: WP_000088046.1
• Coordinates: 20423..21748 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: MJ614_RS00685
• Protein ID: WP_024136327.1
• Coordinates: 21753..22046 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ614_RS00645	16142..16345	-	204	WP_001194555.1	hypothetical protein	-
MJ614_RS00650	16407..17264	-	858	WP_000784387.1	HNH endonuclease signature motif containing protein	-
MJ614_RS00655	17280..17588	-	309	WP_001224687.1	hypothetical protein	-
MJ614_RS00660	18137..18562	-	426	WP_001043843.1	hypothetical protein	-
MJ614_RS00665	18816..19631	-	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
MJ614_RS00670	19644..20054	-	411	WP_000985911.1	hypothetical protein	-
MJ614_RS00675	20156..20362	-	207	WP_000134171.1	hypothetical protein	-
MJ614_RS00680	20423..21748	-	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
MJ614_RS00685	21753..22046	-	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
MJ614_RS00690	22650..23042	-	393	WP_000341066.1	hypothetical protein	-
MJ614_RS00695	23415..23756	+	342	WP_001151575.1	hypothetical protein	-
MJ614_RS00700	23848..24039	+	192	WP_000398480.1	hypothetical protein	-
MJ614_RS00705	24089..24370	+	282	WP_000077926.1	hypothetical protein	-
MJ614_RS26625	24711..24842	-	132	WP_000374059.1	hypothetical protein	-
MJ614_RS00710	24933..26174	-	1242	WP_001053338.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaNDM-5 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / dfrA27 / aadA16	-	1..256955	256955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T236846 WP_000088046.1 NZ_CP092646:c21748-20423 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T236846 NZ_LR740759:158629-158790 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT236846 WP_024136327.1 NZ_CP092646:c22046-21753 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT236846 NZ_LR740759:158365-158576 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1