Detailed information of TA system TA236845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 38729..39330
• Replicon: plasmid pEC5502-2
• Accession: NZ_CP092645
• Organism: Escherichia coli strain EC5502

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1M0S1Q8
• Locus tag: MJ614_RS00195
• Protein ID: WP_024236459.1
• Coordinates: 38729..39109 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: MJ614_RS00200
• Protein ID: WP_001190712.1
• Coordinates: 39109..39330 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ614_RS00165	33829..33948	-	120	WP_071527722.1	ash family protein	-
MJ614_RS00170	34170..35654	-	1485	WP_000124155.1	hypothetical protein	-
MJ614_RS00175	35654..36847	-	1194	WP_089477059.1	terminase	-
MJ614_RS00180	36933..37385	-	453	WP_001326849.1	late promoter-activating protein	-
MJ614_RS00185	37474..38517	-	1044	WP_001561086.1	DUF968 domain-containing protein	-
MJ614_RS00190	38545..38724	-	180	WP_000113018.1	hypothetical protein	-
MJ614_RS00195	38729..39109	-	381	WP_024236459.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MJ614_RS00200	39109..39330	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MJ614_RS00205	39403..39792	-	390	WP_000506727.1	S24 family peptidase	-
MJ614_RS00210	39967..40539	+	573	WP_001133670.1	hypothetical protein	-
MJ614_RS00215	40546..40797	-	252	Protein_42	DNA polymerase III subunit theta	-
MJ614_RS00220	41247..41384	+	138	WP_000123562.1	hypothetical protein	-
MJ614_RS00225	41459..41821	-	363	WP_001261544.1	hypothetical protein	-
MJ614_RS00230	41818..42693	-	876	WP_228637137.1	hypothetical protein	-
MJ614_RS00235	42670..42804	-	135	Protein_46	hypothetical protein	-
MJ614_RS00240	42801..43133	-	333	WP_158212767.1	hypothetical protein	-
MJ614_RS00245	43138..43316	-	179	Protein_48	hypothetical protein	-
MJ614_RS00250	43318..43950	-	633	WP_001289894.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-55	-	1..99284	99284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13643.37 Da
• Isoelectric Point: 5.1514

>T236845 WP_024236459.1 NZ_CP092645:c39109-38729 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T236845 NZ_LR740759:143065-143370 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT236845 WP_001190712.1 NZ_CP092645:c39330-39109 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT236845 NZ_LR740759:142845-143063 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A1M0S1Q8

Antitoxin

• AlphaFold DB: A0A829CJB6