Detailed information of TA system TA236840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3397583..3398420
• Replicon: chromosome
• Accession: NZ_CP092639
• Organism: Escherichia coli strain HS

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: MJ392_RS16440
• Protein ID: WP_000227784.1
• Coordinates: 3397878..3398420 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MJ392_RS16435
• Protein ID: WP_001297137.1
• Coordinates: 3397583..3397894 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ392_RS16410 (3392603)	3392603..3393550	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
MJ392_RS16415 (3393572)	3393572..3395563	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MJ392_RS16420 (3395553)	3395553..3396167	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MJ392_RS16425 (3396167)	3396167..3396496	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MJ392_RS16430 (3396508)	3396508..3397398	+	891	WP_000971336.1	heme o synthase	-
MJ392_RS16435 (3397583)	3397583..3397894	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MJ392_RS16440 (3397878)	3397878..3398420	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
MJ392_RS16445 (3398476)	3398476..3399411	-	936	WP_024186686.1	tetratricopeptide repeat protein	-
MJ392_RS16450 (3399819)	3399819..3401183	+	1365	WP_001000978.1	MFS transporter	-
MJ392_RS16455 (3401311)	3401311..3401802	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
MJ392_RS16460 (3401970)	3401970..3402881	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T236840 WP_000227784.1 NZ_CP092639:3397878-3398420 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T236840 NZ_LR740758:c5014740-5014483 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT236840 WP_001297137.1 NZ_CP092639:3397583-3397894 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT236840 NZ_LR740758:c5014471-5013926 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCGGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCTTTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3