Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3363504..3364122 Replicon chromosome
Accession NZ_CP092639
Organism Escherichia coli strain HS

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MJ392_RS16270 Protein ID WP_001291435.1
Coordinates 3363904..3364122 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MJ392_RS16265 Protein ID WP_000344800.1
Coordinates 3363504..3363878 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ392_RS16255 (3358593) 3358593..3359786 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MJ392_RS16260 (3359809) 3359809..3362958 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MJ392_RS16265 (3363504) 3363504..3363878 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MJ392_RS16270 (3363904) 3363904..3364122 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MJ392_RS16275 (3364294) 3364294..3364845 + 552 WP_000102564.1 maltose O-acetyltransferase -
MJ392_RS16280 (3364961) 3364961..3365431 + 471 WP_000136192.1 YlaC family protein -
MJ392_RS16285 (3365595) 3365595..3367145 + 1551 WP_001529523.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MJ392_RS16290 (3367187) 3367187..3367519 - 333 WP_001741293.1 DUF1428 family protein -
MJ392_RS16300 (3367919) 3367919..3368230 + 312 WP_000409911.1 MGMT family protein -
MJ392_RS16305 (3368261) 3368261..3368833 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236839 WP_001291435.1 NZ_CP092639:3363904-3364122 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T236839 NZ_LR740758:4766583-4766756 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT236839 WP_000344800.1 NZ_CP092639:3363504-3363878 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT236839 NZ_LR740758:4766259-4766555 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References