Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2380280..2380918 Replicon chromosome
Accession NZ_CP092639
Organism Escherichia coli strain HS

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag MJ392_RS11530 Protein ID WP_000813794.1
Coordinates 2380742..2380918 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag MJ392_RS11525 Protein ID WP_001270286.1
Coordinates 2380280..2380696 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ392_RS11505 (2375432) 2375432..2376373 - 942 WP_001251313.1 ABC transporter permease -
MJ392_RS11510 (2376374) 2376374..2377387 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
MJ392_RS11515 (2377405) 2377405..2378550 - 1146 WP_000047421.1 ABC transporter substrate-binding protein -
MJ392_RS11520 (2378795) 2378795..2380201 - 1407 WP_000760619.1 PLP-dependent aminotransferase family protein -
MJ392_RS11525 (2380280) 2380280..2380696 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
MJ392_RS11530 (2380742) 2380742..2380918 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
MJ392_RS11535 (2381140) 2381140..2381370 + 231 WP_000494244.1 YncJ family protein -
MJ392_RS11540 (2381462) 2381462..2383423 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
MJ392_RS11545 (2383496) 2383496..2384032 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
MJ392_RS11550 (2384124) 2384124..2385299 + 1176 WP_001236261.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T236837 WP_000813794.1 NZ_CP092639:c2380918-2380742 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T236837 NZ_LR740758:4454604-4454915 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT236837 WP_001270286.1 NZ_CP092639:c2380696-2380280 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT236837 NZ_LR740758:4454916-4455206 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References