Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 842318..842972 Replicon chromosome
Accession NZ_CP092639
Organism Escherichia coli strain HS

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MJ392_RS04150 Protein ID WP_000244777.1
Coordinates 842565..842972 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MJ392_RS04145 Protein ID WP_000354046.1
Coordinates 842318..842584 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ392_RS04120 (837487) 837487..838230 + 744 WP_000951948.1 SDR family oxidoreductase -
MJ392_RS04125 (838287) 838287..839720 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
MJ392_RS04130 (839765) 839765..840076 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MJ392_RS04135 (840240) 840240..840899 + 660 WP_000250274.1 hemolysin III family protein -
MJ392_RS04140 (841095) 841095..842075 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MJ392_RS04145 (842318) 842318..842584 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MJ392_RS04150 (842565) 842565..842972 + 408 WP_000244777.1 protein YgfX Toxin
MJ392_RS04155 (843012) 843012..843533 - 522 WP_001055874.1 flavodoxin FldB -
MJ392_RS04160 (843645) 843645..844541 + 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
MJ392_RS04165 (844566) 844566..845276 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MJ392_RS04170 (845282) 845282..847015 + 1734 WP_000813207.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T236824 WP_000244777.1 NZ_CP092639:842565-842972 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T236824 NZ_LR740758:1684794-1685072 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT236824 WP_000354046.1 NZ_CP092639:842318-842584 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236824 NZ_LR740758:1685072-1685356 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References