Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4687222..4687738 Replicon chromosome
Accession NZ_CP092635
Organism Enterobacter cloacae strain EFN743

Toxin (Protein)


Gene name relE Uniprot ID A0A7Z1JCT7
Locus tag MKZ88_RS22560 Protein ID WP_058651170.1
Coordinates 4687222..4687506 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7Z1JC92
Locus tag MKZ88_RS22565 Protein ID WP_058651171.1
Coordinates 4687496..4687738 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ88_RS22545 (MKZ88_22545) 4682476..4684119 + 1644 WP_058651167.1 alpha,alpha-phosphotrehalase -
MKZ88_RS22550 (MKZ88_22550) 4684493..4686631 + 2139 WP_058651168.1 anaerobic ribonucleoside-triphosphate reductase -
MKZ88_RS22555 (MKZ88_22555) 4686754..4687218 + 465 WP_058651169.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
MKZ88_RS22560 (MKZ88_22560) 4687222..4687506 - 285 WP_058651170.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MKZ88_RS22565 (MKZ88_22565) 4687496..4687738 - 243 WP_058651171.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MKZ88_RS22570 (MKZ88_22570) 4687816..4689726 - 1911 WP_058651172.1 PRD domain-containing protein -
MKZ88_RS22575 (MKZ88_22575) 4689746..4690882 - 1137 WP_058651173.1 lactonase family protein -
MKZ88_RS22580 (MKZ88_22580) 4691003..4691743 - 741 WP_058651174.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11024.95 Da        Isoelectric Point: 10.4610

>T236819 WP_058651170.1 NZ_CP092635:c4687506-4687222 [Enterobacter cloacae]
ITYELEFDPRVLKEWSKLGETVKKQFRKKLAGVLVNPRIESARLHNLPDCYKIKLRSQGYRLVYQVQDNVVTVVVIAIGK
REKLTVYHDANKRL

Download         Length: 285 bp

>T236819 NZ_LR740758:284315-284713 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8971.39 Da        Isoelectric Point: 6.2984

>AT236819 WP_058651171.1 NZ_CP092635:c4687738-4687496 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDNL

Download         Length: 243 bp

>AT236819 NZ_LR740758:284019-284312 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z1JCT7

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1JC92

References