Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4069405..4070025 Replicon chromosome
Accession NZ_CP092635
Organism Enterobacter cloacae strain EFN743

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag MKZ88_RS19710 Protein ID WP_013095889.1
Coordinates 4069807..4070025 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag MKZ88_RS19705 Protein ID WP_008499288.1
Coordinates 4069405..4069779 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ88_RS19695 (MKZ88_19695) 4064530..4065723 + 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKZ88_RS19700 (MKZ88_19700) 4065746..4068892 + 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit AcrB -
MKZ88_RS19705 (MKZ88_19705) 4069405..4069779 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
MKZ88_RS19710 (MKZ88_19710) 4069807..4070025 + 219 WP_013095889.1 HHA domain-containing protein Toxin
MKZ88_RS19715 (MKZ88_19715) 4070235..4070786 + 552 WP_013095888.1 maltose O-acetyltransferase -
MKZ88_RS19720 (MKZ88_19720) 4070902..4071369 + 468 WP_013095887.1 YlaC family protein -
MKZ88_RS19725 (MKZ88_19725) 4071341..4072801 - 1461 WP_058651634.1 PLP-dependent aminotransferase family protein -
MKZ88_RS19730 (MKZ88_19730) 4072903..4073613 + 711 WP_058651633.1 GNAT family protein -
MKZ88_RS19735 (MKZ88_19735) 4073610..4073750 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MKZ88_RS19740 (MKZ88_19740) 4073753..4074013 - 261 WP_006176933.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T236818 WP_013095889.1 NZ_CP092635:4069807-4070025 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T236818 NZ_LR740758:53260-53382 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT236818 WP_008499288.1 NZ_CP092635:4069405-4069779 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT236818 NZ_LR740758:53024-53257 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References