Detailed information of TA system TA023681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2988775..2989730
• Replicon: chromosome
• Accession: NC_012522
• Organism: Rhodococcus opacus B4

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: C1B596
• Locus tag: ROP_RS13770
• Protein ID: WP_012689978.1
• Coordinates: 2989149..2989730 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: C1B595
• Locus tag: ROP_RS13765
• Protein ID: WP_012689977.1
• Coordinates: 2988775..2989152 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROP_RS13745	2985243..2986412	+	1170	WP_012689973.1	NADH:flavin oxidoreductase	-
ROP_RS13750	2986420..2986908	-	489	WP_012689974.1	SRPBCC family protein	-
ROP_RS13755	2986905..2987237	-	333	WP_012689975.1	metalloregulator ArsR/SmtB family transcription factor	-
ROP_RS13760	2987420..2988700	+	1281	WP_012689976.1	DUF2029 domain-containing protein	-
ROP_RS13765	2988775..2989152	+	378	WP_012689977.1	hypothetical protein	Antitoxin
ROP_RS13770	2989149..2989730	+	582	WP_012689978.1	zeta toxin family protein	Toxin
ROP_RS13775	2989879..2992194	+	2316	WP_012689979.1	serine/threonine protein kinase	-
ROP_RS13780	2992292..2992918	-	627	WP_012689980.1	TetR/AcrR family transcriptional regulator	-
ROP_RS13785	2993028..2994236	+	1209	WP_043824729.1	DUF2236 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (5-133)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-60)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21301.20 Da
• Isoelectric Point: 6.7657

>T23681 WP_012689978.1 NC_012522:2989149-2989730 [Rhodococcus opacus B4]
VRRLDLIVGPNGAGKTTFAEQRLAPLLPGSAFVNADLIAADRWPGSAEQHSYEAARIAATIRDTLIERGESFIAETVFSH
PSKLELIDRARRAGYVVVLHVILVPVELSVARVVERVAEGGHSVPEDKIRERFKRLWPLVAEAIDRSDHATVYDNTAPDV
TRIVARYVDGHEAGKITWPAWTPAELTTQPPRS

Nucleotide

• Length: 582 bp

>T23681 NC_012522:2989149-2989730 [Rhodococcus opacus B4]
GTGAGGCGTCTCGATCTCATCGTCGGGCCCAACGGCGCCGGCAAGACGACCTTCGCCGAACAACGCCTTGCACCGCTCCT
ACCCGGAAGTGCGTTCGTCAATGCCGATTTGATCGCCGCGGACCGGTGGCCGGGCAGCGCCGAACAACACTCGTACGAGG
CCGCGCGGATCGCGGCAACCATCCGCGACACGTTGATCGAGCGTGGCGAGTCGTTCATCGCGGAGACGGTCTTCTCGCAT
CCATCGAAACTCGAACTGATCGACCGGGCGCGACGTGCCGGGTATGTCGTGGTGCTCCACGTGATCCTCGTCCCGGTCGA
GTTGTCGGTAGCCCGTGTGGTCGAACGGGTGGCCGAGGGTGGGCACTCGGTGCCCGAGGACAAGATCCGCGAACGATTCA
AGCGGCTGTGGCCGCTGGTGGCCGAAGCGATCGACCGCTCCGACCACGCAACCGTGTACGACAACACCGCTCCGGACGTG
ACGCGGATCGTCGCACGGTACGTCGACGGACACGAAGCCGGTAAAATCACCTGGCCTGCATGGACTCCGGCGGAGTTGAC
CACGCAACCTCCTCGGTCGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13720.37 Da
• Isoelectric Point: 6.7333

>AT23681 WP_012689977.1 NC_012522:2988775-2989152 [Rhodococcus opacus B4]
MKKIADKVTRFSSDLVDAASTEGERENRSARQQLEHWARVGREVSNQRHVARRRVEAALTGRVPLSELSVEEGVVFNAEI
SAALEESLATGNHVAERAGRGLSTVALDEQGRVVKYLPDGTQILL

Nucleotide

• Length: 378 bp

>AT23681 NC_012522:2988775-2989152 [Rhodococcus opacus B4]
ATGAAGAAAATTGCCGACAAGGTAACCAGATTCTCGTCGGACCTCGTCGATGCCGCGTCGACCGAAGGTGAGCGGGAGAA
TCGCTCCGCTCGCCAGCAGCTCGAACACTGGGCCCGCGTGGGCCGCGAAGTGTCGAATCAGCGTCACGTGGCACGTCGCC
GCGTGGAGGCCGCTCTCACCGGACGAGTGCCGTTGAGCGAGCTGTCCGTCGAGGAAGGGGTAGTGTTCAACGCCGAGATC
TCGGCGGCGCTCGAAGAATCGTTGGCCACCGGCAATCACGTTGCTGAACGCGCAGGTCGGGGGCTGTCAACGGTCGCGCT
CGACGAGCAAGGCCGCGTCGTCAAGTACCTGCCTGACGGTACGCAGATTCTTCTGTGA

Structures

Toxin

• AlphaFold DB: C1B596

Antitoxin

• AlphaFold DB: C1B595