Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 133536..135159 Replicon chromosome
Accession NZ_CP092635
Organism Enterobacter cloacae strain EFN743

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag MKZ88_RS00730 Protein ID WP_000088044.1
Coordinates 133834..135159 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag MKZ88_RS00725 Protein ID WP_012477377.1
Coordinates 133536..133829 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ88_RS00705 (MKZ88_00705) 129335..129727 + 393 WP_000341066.1 hypothetical protein -
MKZ88_RS00710 (MKZ88_00710) 130386..131042 + 657 WP_249084045.1 hypothetical protein -
MKZ88_RS00715 (MKZ88_00715) 131245..131742 + 498 WP_000340139.1 membrane protein -
MKZ88_RS00720 (MKZ88_00720) 131747..133135 + 1389 WP_000083579.1 hypothetical protein -
MKZ88_RS00725 (MKZ88_00725) 133536..133829 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
MKZ88_RS00730 (MKZ88_00730) 133834..135159 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
MKZ88_RS00735 (MKZ88_00735) 135220..135426 + 207 WP_000134171.1 hypothetical protein -
MKZ88_RS00740 (MKZ88_00740) 135527..135937 + 411 WP_000985909.1 hypothetical protein -
MKZ88_RS00745 (MKZ88_00745) 135950..136765 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
MKZ88_RS00750 (MKZ88_00750) 137023..137727 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
MKZ88_RS00755 (MKZ88_00755) 138549..139190 - 642 WP_013851373.1 tetracycline resistance transcriptional repressor TetR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island dfrA14 / qnrB1 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) - 67817..140517 72700


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T236803 WP_000088044.1 NZ_CP092635:133834-135159 [Enterobacter cloacae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T236803 NZ_LR739069:c957470-957288 [Pseudomonas aeruginosa]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAGGGAAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT236803 WP_012477377.1 NZ_CP092635:133536-133829 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT236803 NZ_LR739069:c957241-956813 [Pseudomonas aeruginosa]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTCGCCGTTTTCTGCAGAGACCTGCCGGAACT
GAACAGCTACGGCGACGATAAGGCCCATGCAATCAGCGAGGCGGTTGACGCCATCGAGTCAACCCTCTCGCTGTACGTTG
ATCAGCGCCGAGAAATCCCCGCAGCCAGCCAGGCACAACCAGGCGAGCGCGTTATCCATCTGCCGGCGGTTACCGTTGCG
AAGGTCGCGCTCTGGAACGAAATGATCCGTCGAGATATGCGAAAAGCTGACCTCTGCCGGCTCCTCGGGATCGCGCAGAT
CCAGGGTGACAGGCTCGTCGACTTCCTCCACAACACCAAGATGGAAGCCATGGAGAACGCGCTATCCGCCCTCGGCCTCC
GCCTATCAGTGAATATCGAGGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References