Detailed information of TA system TA023680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 510213..510866
• Replicon: chromosome
• Accession: NC_012491
• Organism: Brevibacillus brevis NBRC 100599

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: J2PLP3
• Locus tag: BBR47_RS02790
• Protein ID: WP_005830205.1
• Coordinates: 510516..510866 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2GZH3
• Locus tag: BBR47_RS02785
• Protein ID: WP_007721089.1
• Coordinates: 510213..510512 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBR47_RS02770	506778..508151	+	1374	WP_012684241.1	hypothetical protein	-
BBR47_RS02775	508337..508777	+	441	WP_012684242.1	hypothetical protein	-
BBR47_RS02780	508780..509982	+	1203	WP_012684243.1	alanine racemase	-
BBR47_RS02785	510213..510512	+	300	WP_007721089.1	CopG family ribbon-helix-helix protein	Antitoxin
BBR47_RS02790	510516..510866	+	351	WP_005830205.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BBR47_RS02795	511075..511503	+	429	WP_012684244.1	anti-sigma regulatory factor	-
BBR47_RS02800	511509..512510	+	1002	WP_012684245.1	PP2C family protein-serine/threonine phosphatase	-
BBR47_RS02805	512536..512862	+	327	WP_012684246.1	anti-sigma factor antagonist	-
BBR47_RS02810	512862..513356	+	495	WP_012684247.1	anti-sigma B factor RsbW	-
BBR47_RS02815	513325..514113	+	789	WP_012684248.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12917.86 Da
• Isoelectric Point: 4.8435

>T23680 WP_005830205.1 NC_012491:510516-510866 [Brevibacillus brevis NBRC 100599]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T23680 NC_012491:510516-510866 [Brevibacillus brevis NBRC 100599]
GTGATTGTCAAACGTGGCGATGTGTTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACGGCGCAGATTCAAAAGG
CGAAGCTGCCTACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTTATTCTGTTGGAACAAATA
CGAACCATTGACAAACAACGTCTAACTGATAAAATTACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
ACAAATCAGTTTAGGACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11309.19 Da
• Isoelectric Point: 10.2101

>AT23680 WP_007721089.1 NC_012491:510213-510512 [Brevibacillus brevis NBRC 100599]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT23680 NC_012491:510213-510512 [Brevibacillus brevis NBRC 100599]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGTCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAACGTACAATCCGTGAGACCATGCAAAAAGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAAGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: J2PLP3

Antitoxin

• AlphaFold DB: J2GZH3