Detailed information of TA system TA236796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 152038..152543
• Replicon: chromosome
• Accession: NZ_CP092634
• Organism: Pseudomonas aeruginosa strain LS.2c

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: MKK05_RS00690
• Protein ID: WP_003083773.1
• Coordinates: 152038..152319 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: MKK05_RS00695
• Protein ID: WP_003083775.1
• Coordinates: 152316..152543 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKK05_RS00665 (MKK05_00665)	147289..148638	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
MKK05_RS00670 (MKK05_00670)	148687..149373	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
MKK05_RS00675 (MKK05_00675)	149474..150208	+	735	WP_003128333.1	GntR family transcriptional regulator	-
MKK05_RS00680 (MKK05_00680)	150388..150798	+	411	WP_003110659.1	aegerolysin family protein	-
MKK05_RS00685 (MKK05_00685)	150830..151738	-	909	WP_003083769.1	LysR family transcriptional regulator	-
MKK05_RS00690 (MKK05_00690)	152038..152319	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
MKK05_RS00695 (MKK05_00695)	152316..152543	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
MKK05_RS00700 (MKK05_00700)	152719..153339	-	621	WP_003101226.1	hypothetical protein	-
MKK05_RS00705 (MKK05_00705)	153440..153940	+	501	WP_003101228.1	LEA type 2 family protein	-
MKK05_RS00710 (MKK05_00710)	154013..154354	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
MKK05_RS00715 (MKK05_00715)	154436..155863	-	1428	WP_003083784.1	GABA permease	-
MKK05_RS00720 (MKK05_00720)	156032..157525	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T236796 WP_003083773.1 NZ_CP092634:c152319-152038 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T236796 NZ_LR739068:c148268-147987 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACTTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT236796 WP_003083775.1 NZ_CP092634:c152543-152316 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT236796 NZ_LR739068:c148492-148265 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9