Detailed information of TA system TA236793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 28538..29274
• Replicon: plasmid pML9ba2
• Accession: NZ_CP092633
• Organism: Klebsiella variicola subsp. variicola strain ML.9ba2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MKK01_RS26595
• Protein ID: WP_240289783.1
• Coordinates: 28792..29274 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MKK01_RS26590
• Protein ID: WP_003026799.1
• Coordinates: 28538..28804 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKK01_RS26565 (MKK01_26565)	24749..25324	-	576	WP_240289780.1	recombinase family protein	-
MKK01_RS26570 (MKK01_26570)	25535..26008	-	474	WP_013213991.1	GNAT family N-acetyltransferase	-
MKK01_RS26575 (MKK01_26575)	26446..27141	+	696	Protein_20	IS21-like element ISSen3 family helper ATPase IstB	-
MKK01_RS26580 (MKK01_26580)	27293..27997	-	705	WP_240289781.1	IS6 family transposase	-
MKK01_RS26585 (MKK01_26585)	28124..28297	+	174	WP_275892396.1	cell envelope integrity TolA C-terminal domain-containing protein	-
MKK01_RS26590 (MKK01_26590)	28538..28804	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MKK01_RS26595 (MKK01_26595)	28792..29274	+	483	WP_240289783.1	GNAT family N-acetyltransferase	Toxin
MKK01_RS26600 (MKK01_26600)	29500..29684	-	185	Protein_25	IS3 family transposase	-
MKK01_RS26605 (MKK01_26605)	29959..31122	-	1164	WP_240289784.1	response regulator	-
MKK01_RS26610 (MKK01_26610)	31115..31579	-	465	WP_240289785.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..166327	166327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17267.98 Da
• Isoelectric Point: 9.5893

>T236793 WP_240289783.1 NZ_CP092633:28792-29274 [Klebsiella variicola subsp. variicola]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHKRTLFLKLP

Nucleotide

• Length: 483 bp

>T236793 NZ_LR738724:c1918802-1918467 [Streptococcus suis]
TTGGATAAGTACCAAGTAAACCTACCTCTAGCTATATATGAGGAACTAGCTGACATTCGCAGCTACATTAGGGAAGAACT
AAAAAGCCCTGATGCTGCGGACAAAAAAATCCAAGAGCTAATAGCAGGACTTAGAAGCCTTGAAATTTTCCCAGAGCGGG
GTTTTAACGTAGATGAACGCTCTAAAAAGGGACAGCTAGTCCCAGGTCAGCTGACGCGAGGACTACCAATCAAAAAGGAT
TATATAGCTATTTATAACATTGATGAAGCACAAAAGGTCGTAAACGTTCGCTATCTTGTCGCAAGCAAGAGCGACTATAT
GAGATTGTTCAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT236793 WP_003026799.1 NZ_CP092633:28538-28804 [Klebsiella variicola subsp. variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT236793 NZ_LR738724:c1919082-1918795 [Streptococcus suis]
ATGGCCTTAACAGCTAAAAAGAAATTGATTAACTTTCAATCAGAAAAATCACTTGTAGAAGAAGCAAAAGTGGTCTTGGC
AAGTCAAAATATGACCATGACTCAAGCTCTCAACCTGTTTCTCAAAAATATTGTAGTAACAGGAGAAATTGGGCTTAAAA
GTGAAGAAGAACTGGAAAAAGAGCGACTATTTGCCGAACTGCAAGCAGAAGTGATGAAAAATGTAGCTGAAATGAATGCG
GGCAACTATCTATCATTGGACGAAATGGAGACCAAGCTCTTTGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66