Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1780291..1780881 Replicon chromosome
Accession NZ_CP092632
Organism Klebsiella variicola subsp. variicola strain ML.9ba2

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag MKK01_RS08535 Protein ID WP_008804165.1
Coordinates 1780549..1780881 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag MKK01_RS08530 Protein ID WP_012541132.1
Coordinates 1780291..1780548 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKK01_RS08510 (MKK01_08510) 1775898..1776473 + 576 WP_012541129.1 hypothetical protein -
MKK01_RS08515 (MKK01_08515) 1776652..1777488 + 837 WP_008804155.1 alpha/beta hydrolase -
MKK01_RS08520 (MKK01_08520) 1777695..1778666 + 972 WP_048293502.1 sensor domain-containing diguanylate cyclase -
MKK01_RS08525 (MKK01_08525) 1778663..1779763 - 1101 WP_240289664.1 phosphotransferase -
MKK01_RS08530 (MKK01_08530) 1780291..1780548 + 258 WP_012541132.1 antitoxin Antitoxin
MKK01_RS08535 (MKK01_08535) 1780549..1780881 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKK01_RS08545 (MKK01_08545) 1781204..1782640 + 1437 WP_164728680.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
MKK01_RS08555 (MKK01_08555) 1783012..1784466 - 1455 WP_240289665.1 AMP nucleosidase -
MKK01_RS08560 (MKK01_08560) 1784597..1784842 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T236781 WP_008804165.1 NZ_CP092632:1780549-1780881 [Klebsiella variicola subsp. variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T236781 NZ_LR738722:c1955022-1954693 [Streptococcus suis]
ATGGAATTTGAGTCTTATTCAGTGATACTTGCTCCAGCAGTGGAGAAGGAATTGGCAGTTATTTACGCCTATTTTTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTCGAAGCATTGGAGAGTTTGCAAATTTTTCCTGAAC
GTGGTTTTAACGCCGATAACCGTTTTGGTAAGCAGATAGACCCGCCTCATCTAACCCGAGGCTATGTCGTTGGAAAAGAC
TACATCGCCCTCTATCGCGTAGTGAAAAATGAAGTTCGAGTTGGTCATCTTTTTGCGACCAAGAGTGATTATGTGAAGTT
GTTGAAGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT236781 WP_012541132.1 NZ_CP092632:1780291-1780548 [Klebsiella variicola subsp. variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT236781 NZ_LR738722:c1955299-1955012 [Streptococcus suis]
TTGATAACTATAAATAAAGATGCCCAAGTAAATTTTAAAACTGATTCCAGACTTTTAGAAAAAGCCAAGGAAGTATTTGC
TATGAACCAGCTGGATTTGACTGCTGGTTTTAACCTCTTCTTGCAAAATATCGCTGTAAAAAATGAGTTGCCAATTCTGA
CAGAAGAAGAGTTGGAAAGAGAAGAGTTGTTTTTACAGTTACAGAAGGAAATCCAGGAAAACCAACAGGCGATTGAGGCA
GGTCACGGGATCAGCCTAGAAAGTGTCAAAGAGAAGTATGGAATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References