Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 329543..330129 Replicon chromosome
Accession NZ_CP092632
Organism Klebsiella variicola subsp. variicola strain ML.9ba2

Toxin (Protein)


Gene name doc Uniprot ID A0A2W7TCK5
Locus tag MKK01_RS01505 Protein ID WP_008806973.1
Coordinates 329761..330129 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MKK01_RS01500 Protein ID WP_004174006.1
Coordinates 329543..329764 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKK01_RS01480 (MKK01_01480) 325699..326625 + 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MKK01_RS01485 (MKK01_01485) 326622..327899 + 1278 WP_110234087.1 branched chain amino acid ABC transporter permease LivM -
MKK01_RS01490 (MKK01_01490) 327896..328663 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MKK01_RS01495 (MKK01_01495) 328665..329378 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MKK01_RS01500 (MKK01_01500) 329543..329764 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MKK01_RS01505 (MKK01_01505) 329761..330129 + 369 WP_008806973.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MKK01_RS01510 (MKK01_01510) 330406..331722 + 1317 WP_022066271.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MKK01_RS01515 (MKK01_01515) 331829..332716 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MKK01_RS01520 (MKK01_01520) 332713..333558 + 846 WP_015369329.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MKK01_RS01525 (MKK01_01525) 333560..334630 + 1071 WP_008806977.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 326622..338276 11654


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(6-116)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13639.01 Da        Isoelectric Point: 7.3191

>T236777 WP_008806973.1 NZ_CP092632:329761-330129 [Klebsiella variicola subsp. variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLVISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T236777 NZ_LR738722:769547-770323 [Streptococcus suis]
ATGAGATTGGAAGAATTTAGTGAGGAAGAGTTTCAGAAGGCCTTGCAGCGAACTATTAGGGCCCAAAGTCGTGGGAAGAC
GATTCCAAATCAACCGAGAGCCATTTTACTTGGAGGTCAGAGCGGAGCAGGTAAGACGACTATTCATCGAATTAAACAAA
AAGAATTTCAAGGTAATATCATTATCATCGATGGTGATAGCTACCGTTCTCAGCATCCCAATTACTTAGCCTTGCAAGAT
AAGTATGGCAAGGACAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAGCATCTGGTTGACGAACTCAGCAC
GCAGGGTTATCATTTGTTAATTGAAGGGACTTTGCGTACCACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGCACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAA
CTTTATGCCATCGATCCCAATCAGGCTAGAGCAACACCAAAAGAACACCATGATGGGATTGTAGAGAACTTGGTTGATAA
CCTAAAGGAGCTTGAAAGCGATAAACTCTTTGACCAAATTCAGATTTATCAAAGAGATCGAACTTGTATCTACGATTCTG
AAACTGATGAAGGCTCCGCAGCAGAGGTTCTTCAAAACTGTCTTTTCGGGAAGTGGAGTAAGGTTGAGGAAGAGATGTTG
AAGGTAGGACAGGAACGGTTGGGGGAATTAGTTTCAGCTAATGGAGAGAAGAAATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT236777 WP_004174006.1 NZ_CP092632:329543-329764 [Klebsiella variicola subsp. variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT236777 NZ_LR738722:769071-769547 [Streptococcus suis]
ATGATTGGAGACAACATCAAGTCACTACGTCGGACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCTTAAGTCGTTATGAAAATGGAACCAGCACCGTATCAACAGAACTCATCGACCGCATTTGTCAAAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTTGAAGATTACCAACTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCACAGCTTTATAGATACCAAGATAGTCAAGACATCGCCTTTGATGATGA
ATCGAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATTAACACGAAAATTTACCTTGTCGATACCTTCGATG
AAATAGAGCGCTACAATGGCTATTTAGACGGTATCGAGCGGATGTTAGACATGGTGCATCATTGGGTGGTGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W7TCK5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References