Detailed information of TA system TA236766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1329126..1330042
• Replicon: chromosome
• Accession: NZ_CP092631
• Organism: Bacillus subtilis strain YB-15

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MKS87_RS06980
• Protein ID: WP_089172519.1
• Coordinates: 1329296..1330042 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: MKS87_RS06975
• Protein ID: WP_003232646.1
• Coordinates: 1329126..1329296 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKS87_RS06940 (1325992)	1325992..1326318	+	327	WP_173614041.1	XkdW family protein	-
MKS87_RS06945 (1326315)	1326315..1326479	+	165	WP_134155238.1	XkdX family protein	-
MKS87_RS06950 (1326523)	1326523..1327362	+	840	WP_173614042.1	phage-like element PBSX protein XepA	-
MKS87_RS06955 (1327415)	1327415..1327684	+	270	WP_003232655.1	hemolysin XhlA family protein	-
MKS87_RS06960 (1327697)	1327697..1327960	+	264	WP_173614043.1	phage holin	-
MKS87_RS06965 (1327973)	1327973..1328866	+	894	WP_029317679.1	N-acetylmuramoyl-L-alanine amidase	-
MKS87_RS06970 (1328903)	1328903..1329040	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
MKS87_RS06975 (1329126)	1329126..1329296	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
MKS87_RS06980 (1329296)	1329296..1330042	-	747	WP_089172519.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
MKS87_RS06985 (1330152)	1330152..1331153	-	1002	WP_003232642.1	inorganic phosphate transporter	-
MKS87_RS06990 (1331166)	1331166..1331783	-	618	WP_003218470.1	DUF47 domain-containing protein	-
MKS87_RS06995 (1332059)	1332059..1333375	-	1317	WP_029317680.1	serine/threonine exchanger	-
MKS87_RS07000 (1333764)	1333764..1334714	+	951	WP_014476561.1	ring-cleaving dioxygenase	-
MKS87_RS07005 (1334823)	1334823..1334918	+	96	Protein_1318	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29121.60 Da
• Isoelectric Point: 4.6191

>T236766 WP_089172519.1 NZ_CP092631:c1330042-1329296 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMFAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T236766 NZ_LR738720:798335-798604 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT236766 WP_003232646.1 NZ_CP092631:c1329296-1329126 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT236766 NZ_LR738720:798118-798345 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGATTAAACGATGCAGAAGAAGAAATTTTCAGAAGCTATGCTATTCATACAGGAAAAAGTCT
GTCTGAGCTATTCAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Structures

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8