Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 5950449..5951957 Replicon chromosome
Accession NZ_CP092629
Organism Pseudomonas aeruginosa strain HU20

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag MKF35_RS27610 Protein ID WP_003119682.1
Coordinates 5951370..5951957 (-) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9HYX2
Locus tag MKF35_RS27605 Protein ID WP_003115566.1
Coordinates 5950449..5951303 (-) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKF35_RS27590 5945867..5946076 + 210 WP_003115568.1 cold-shock protein -
MKF35_RS27595 5946138..5948012 - 1875 WP_023128709.1 MFS transporter -
MKF35_RS27600 5948187..5950352 - 2166 WP_047105105.1 TonB-dependent receptor family protein -
MKF35_RS27605 5950449..5951303 - 855 WP_003115566.1 AraC family transcriptional regulator Antitoxin
MKF35_RS27610 5951370..5951957 - 588 WP_003119682.1 GNAT family protein Toxin
MKF35_RS27615 5952223..5955702 + 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T236759 WP_003119682.1 NZ_CP092629:c5951957-5951370 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T236759 NZ_LR735440:1038282-1038461 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31933.23 Da        Isoelectric Point: 7.3276

>AT236759 WP_003115566.1 NZ_CP092629:c5951303-5950449 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Download         Length: 855 bp

>AT236759 NZ_LR735440:1037659-1038258 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAATTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCATCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB Q9HYX2

References