Detailed information of TA system TA236757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3363012..3364269
• Replicon: chromosome
• Accession: NZ_CP092629
• Organism: Pseudomonas aeruginosa strain HU20

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MKF35_RS15625
• Protein ID: WP_003449003.1
• Coordinates: 3363514..3364269 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: MKF35_RS15620
• Protein ID: WP_003086329.1
• Coordinates: 3363012..3363380 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKF35_RS15600	3359175..3359450	+	276	WP_010792073.1	zinc ribbon domain-containing protein	-
MKF35_RS15605	3359453..3359929	-	477	WP_003112531.1	hypothetical protein	-
MKF35_RS15610	3360104..3361597	+	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
MKF35_RS15615	3361653..3362939	+	1287	WP_003112529.1	FAD-binding oxidoreductase	-
MKF35_RS15620	3363012..3363380	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
MKF35_RS15625	3363514..3364269	+	756	WP_003449003.1	RES family NAD+ phosphorylase	Toxin
MKF35_RS15630	3365037..3366398	+	1362	WP_003081943.1	DNA recombination protein RmuC	-
MKF35_RS15635	3366553..3369096	-	2544	WP_003140632.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28292.45 Da
• Isoelectric Point: 7.7294

>T236757 WP_003449003.1 NZ_CP092629:3363514-3364269 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T236757 NZ_LR735440:889174-889383 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT236757 WP_003086329.1 NZ_CP092629:3363012-3363380 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT236757 NZ_LR735440:888874-889167 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7