Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1859..2502 Replicon plasmid p4
Accession NZ_CP092593
Organism Klebsiella pneumoniae strain EFN 299

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MKT41_RS27780 Protein ID WP_001044770.1
Coordinates 1859..2275 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MKT41_RS27785 Protein ID WP_001261282.1
Coordinates 2272..2502 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKT41_RS27765 (MKT41_27755) 136..327 + 192 Protein_1 nucleotidyltransferase domain-containing protein -
MKT41_RS27775 (MKT41_27765) 1096..1785 - 690 Protein_3 AAA family ATPase -
MKT41_RS27780 (MKT41_27770) 1859..2275 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MKT41_RS27785 (MKT41_27775) 2272..2502 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MKT41_RS27790 (MKT41_27780) 2459..2686 + 228 Protein_6 hypothetical protein -
MKT41_RS27795 (MKT41_27785) 3013..3465 - 453 WP_004201034.1 hypothetical protein -
MKT41_RS27800 (MKT41_27790) 4273..4623 + 351 WP_000493378.1 hypothetical protein -
MKT41_RS27805 (MKT41_27795) 4674..5417 + 744 WP_000129823.1 hypothetical protein -
MKT41_RS27810 (MKT41_27800) 5414..6190 + 777 WP_000015958.1 site-specific integrase -
MKT41_RS27815 (MKT41_27805) 6248..6505 - 258 WP_000764642.1 hypothetical protein -
MKT41_RS27820 (MKT41_27810) 6634..6738 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / dfrA14 / catA2 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / qnrB9 - 1..65919 65919


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T236731 WP_001044770.1 NZ_CP092593:c2275-1859 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T236731 NZ_LR699570:3738741-3738998 [Mycobacterium tuberculosis variant bovis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTAGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT236731 WP_001261282.1 NZ_CP092593:c2502-2272 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT236731 NZ_LR699570:3738469-3738744 [Mycobacterium tuberculosis variant bovis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References