Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 143974..144710 Replicon plasmid p1
Accession NZ_CP092590
Organism Klebsiella pneumoniae strain EFN 299

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MKT41_RS26710 Protein ID WP_003026803.1
Coordinates 144228..144710 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MKT41_RS26705 Protein ID WP_003026799.1
Coordinates 143974..144240 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKT41_RS26680 (MKT41_26670) 139065..139412 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
MKT41_RS26685 (MKT41_26675) 139461..140999 + 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
MKT41_RS26690 (MKT41_26680) 141898..142206 - 309 WP_017896554.1 hypothetical protein -
MKT41_RS26695 (MKT41_26685) 142700..143248 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
MKT41_RS26700 (MKT41_26690) 143295..143729 + 435 WP_001567367.1 cell envelope integrity protein TolA -
MKT41_RS26705 (MKT41_26695) 143974..144240 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MKT41_RS26710 (MKT41_26700) 144228..144710 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MKT41_RS26715 (MKT41_26705) 144911..146314 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
MKT41_RS26720 (MKT41_26710) 146343..146975 - 633 WP_001567369.1 hypothetical protein -
MKT41_RS26725 (MKT41_26715) 147194..148597 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
MKT41_RS26730 (MKT41_26720) 148626..149258 - 633 WP_001567369.1 hypothetical protein -
MKT41_RS26735 (MKT41_26725) 149484..149675 + 192 Protein_150 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) - 1..177059 177059
- inside IScluster/Tn - - 138664..148597 9933


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T236728 WP_003026803.1 NZ_CP092590:144228-144710 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T236728 NZ_LR699570:3152038-3152481 [Mycobacterium tuberculosis variant bovis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT236728 WP_003026799.1 NZ_CP092590:143974-144240 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT236728 NZ_LR699570:3151794-3152051 [Mycobacterium tuberculosis variant bovis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References