Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5214005..5214630 Replicon chromosome
Accession NZ_CP092589
Organism Klebsiella pneumoniae strain EFN 299

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MKT41_RS25615 Protein ID WP_002882817.1
Coordinates 5214005..5214388 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MKT41_RS25620 Protein ID WP_004150355.1
Coordinates 5214388..5214630 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKT41_RS25600 (MKT41_25590) 5211371..5212273 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MKT41_RS25605 (MKT41_25595) 5212270..5212905 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MKT41_RS25610 (MKT41_25600) 5212902..5213831 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MKT41_RS25615 (MKT41_25605) 5214005..5214388 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MKT41_RS25620 (MKT41_25610) 5214388..5214630 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MKT41_RS25625 (MKT41_25615) 5214835..5215752 + 918 WP_002882812.1 alpha/beta hydrolase -
MKT41_RS25630 (MKT41_25620) 5215766..5216707 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MKT41_RS25635 (MKT41_25625) 5216752..5217189 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MKT41_RS25640 (MKT41_25630) 5217186..5218046 - 861 WP_002882807.1 virulence factor BrkB family protein -
MKT41_RS25645 (MKT41_25635) 5218040..5218639 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T236727 WP_002882817.1 NZ_CP092589:c5214388-5214005 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T236727 NZ_LR699570:3146478-3146741 [Mycobacterium tuberculosis variant bovis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT236727 WP_004150355.1 NZ_CP092589:c5214630-5214388 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT236727 NZ_LR699570:3146193-3146474 [Mycobacterium tuberculosis variant bovis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References