Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2671387..2672975 Replicon chromosome
Accession NZ_CP092589
Organism Klebsiella pneumoniae strain EFN 299

Toxin (Protein)


Gene name hipA Uniprot ID A0A0H3GN97
Locus tag MKT41_RS13245 Protein ID WP_004199851.1
Coordinates 2671387..2672709 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag MKT41_RS13250 Protein ID WP_002904790.1
Coordinates 2672709..2672975 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKT41_RS13230 (MKT41_13225) 2666452..2668953 + 2502 WP_004199853.1 fimbria/pilus outer membrane usher protein -
MKT41_RS13235 (MKT41_13230) 2668941..2669945 + 1005 WP_004199852.1 fimbrial protein -
MKT41_RS13240 (MKT41_13235) 2670078..2671370 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
MKT41_RS13245 (MKT41_13240) 2671387..2672709 - 1323 WP_004199851.1 type II toxin-antitoxin system HipA family toxin Toxin
MKT41_RS13250 (MKT41_13245) 2672709..2672975 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
MKT41_RS13255 (MKT41_13250) 2673241..2673567 - 327 WP_002904788.1 hydroxyisourate hydrolase -
MKT41_RS13260 (MKT41_13255) 2673564..2674064 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
MKT41_RS13265 (MKT41_13260) 2674042..2675421 - 1380 WP_004199850.1 nucleobase:cation symporter-2 family protein -
MKT41_RS13270 (MKT41_13265) 2675767..2676921 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
MKT41_RS13275 (MKT41_13270) 2676902..2677825 - 924 WP_004199849.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49128.44 Da        Isoelectric Point: 7.2487

>T236722 WP_004199851.1 NZ_CP092589:c2672709-2671387 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T236722 NZ_LR699570:c3037546-3037130 [Mycobacterium tuberculosis variant bovis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT236722 WP_002904790.1 NZ_CP092589:c2672975-2672709 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT236722 NZ_LR699570:c3037809-3037543 [Mycobacterium tuberculosis variant bovis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GN97

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References