Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2097764..2098278 Replicon chromosome
Accession NZ_CP092581
Organism Staphylococcus aureus strain VRMSSA-WC111

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI52_RS10415 Protein ID WP_000581792.1
Coordinates 2097764..2097973 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI52_RS10420 Protein ID WP_000138932.1
Coordinates 2097985..2098278 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI52_RS10395 (MKI52_002059) 2093272..2094630 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI52_RS10400 (MKI52_002060) 2094645..2095715 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI52_RS10405 (MKI52_002061) 2096033..2097235 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI52_RS10410 (MKI52_002062) 2097499..2097636 - 138 WP_000828354.1 Lmo0850 family protein -
MKI52_RS10415 (MKI52_002063) 2097764..2097973 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI52_RS10420 (MKI52_002064) 2097985..2098278 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI52_RS10425 (MKI52_002065) 2098444..2099937 + 1494 WP_000571554.1 cardiolipin synthase -
MKI52_RS10430 (MKI52_002066) 2099965..2100612 + 648 WP_001187633.1 HD domain-containing protein -
MKI52_RS10435 (MKI52_002067) 2101525..2102397 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI52_RS10440 (MKI52_002068) 2102483..2103124 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236699 WP_000581792.1 NZ_CP092581:c2097973-2097764 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236699 NZ_LR699570:c1389439-1389146 [Mycobacterium tuberculosis variant bovis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236699 WP_000138932.1 NZ_CP092581:c2098278-2097985 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236699 NZ_LR699570:c1389705-1389436 [Mycobacterium tuberculosis variant bovis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References