Detailed information of TA system TA236698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2076505..2077034
• Replicon: chromosome
• Accession: NZ_CP092581
• Organism: Staphylococcus aureus strain VRMSSA-WC111

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI52_RS10315
• Protein ID: WP_000621175.1
• Coordinates: 2076505..2076867 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI52_RS10320
• Protein ID: WP_000948331.1
• Coordinates: 2076864..2077034 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI52_RS10295 (MKI52_002040)	2073483..2074253	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI52_RS10300 (MKI52_002041)	2074228..2074707	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI52_RS10305 (MKI52_002042)	2074709..2075035	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI52_RS10310 (MKI52_002043)	2075154..2076155	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI52_RS10315 (MKI52_002044)	2076505..2076867	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI52_RS10320 (MKI52_002045)	2076864..2077034	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI52_RS10325 (MKI52_002046)	2077119..2078267	-	1149	WP_001281154.1	alanine racemase	-
MKI52_RS10330 (MKI52_002047)	2078333..2078692	-	360	WP_000581197.1	holo-ACP synthase	-
MKI52_RS10335 (MKI52_002048)	2078696..2079187	-	492	WP_001205912.1	PH domain-containing protein	-
MKI52_RS10340 (MKI52_002049)	2079174..2080757	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI52_RS10345 (MKI52_002050)	2080750..2081229	-	480	WP_001287087.1	hypothetical protein	-
MKI52_RS10350 (MKI52_002051)	2081437..2081997	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236698 WP_000621175.1 NZ_CP092581:c2076867-2076505 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236698 NZ_LR699570:1384997-1385428 [Mycobacterium tuberculosis variant bovis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236698 WP_000948331.1 NZ_CP092581:c2077034-2076864 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236698 NZ_LR699570:1384740-1385000 [Mycobacterium tuberculosis variant bovis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1