Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1863606..1864382 Replicon chromosome
Accession NZ_CP092581
Organism Staphylococcus aureus strain VRMSSA-WC111

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag MKI52_RS08945 Protein ID WP_000031108.1
Coordinates 1863606..1863758 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag MKI52_RS08950 Protein ID WP_001251224.1
Coordinates 1863783..1864382 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI52_RS08930 (MKI52_001772) 1859808..1860629 + 822 WP_000669376.1 RluA family pseudouridine synthase -
MKI52_RS08935 (MKI52_001773) 1861082..1862467 - 1386 WP_000116228.1 class II fumarate hydratase -
MKI52_RS08940 (MKI52_001774) 1862663..1863058 - 396 WP_000901023.1 hypothetical protein -
MKI52_RS08945 (MKI52_001775) 1863606..1863758 - 153 WP_000031108.1 SAS053 family protein Toxin
MKI52_RS08950 (MKI52_001776) 1863783..1864382 - 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
MKI52_RS08955 (MKI52_001777) 1864541..1865011 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
MKI52_RS08960 (MKI52_001778) 1865016..1866143 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
MKI52_RS08965 (MKI52_001779) 1866294..1867016 - 723 WP_000590809.1 amino acid ABC transporter ATP-binding protein -
MKI52_RS08970 (MKI52_001780) 1867009..1868466 - 1458 WP_000649908.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T236696 WP_000031108.1 NZ_CP092581:c1863758-1863606 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T236696 NZ_LR699570:c1231466-1231155 [Mycobacterium tuberculosis variant bovis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT236696 WP_001251224.1 NZ_CP092581:c1864382-1863783 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT236696 NZ_LR699570:c1231786-1231466 [Mycobacterium tuberculosis variant bovis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References