Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Xre-MNT/HTH_26(antitoxin) |
| Location | 59373..60481 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP092578 | ||
| Organism | Enterococcus faecalis strain VRE-WC031 | ||
Toxin (Protein)
| Gene name | MNTss | Uniprot ID | R3JIN1 |
| Locus tag | MKI49_RS15770 | Protein ID | WP_000233000.1 |
| Coordinates | 59612..60481 (+) | Length | 290 a.a. |
Antitoxin (Protein)
| Gene name | Xress | Uniprot ID | - |
| Locus tag | MKI49_RS15765 | Protein ID | WP_000205227.1 |
| Coordinates | 59373..59597 (+) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKI49_RS15730 (MKI49_003127) | 54962..55132 | + | 171 | WP_000713595.1 | hypothetical protein | - |
| MKI49_RS15735 (MKI49_003128) | 55146..55748 | + | 603 | WP_001062586.1 | recombinase family protein | - |
| MKI49_RS15740 (MKI49_003129) | 55764..56441 | + | 678 | Protein_69 | DNA topoisomerase | - |
| MKI49_RS15745 | 56752..56919 | + | 168 | WP_000898188.1 | hypothetical protein | - |
| MKI49_RS15750 (MKI49_003130) | 56923..57249 | + | 327 | WP_000392725.1 | hypothetical protein | - |
| MKI49_RS15755 (MKI49_003131) | 57250..57666 | + | 417 | WP_000323438.1 | recombinase | - |
| MKI49_RS15760 (MKI49_003132) | 57668..59230 | + | 1563 | WP_000136908.1 | recombinase family protein | - |
| MKI49_RS15765 (MKI49_003133) | 59373..59597 | + | 225 | WP_000205227.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| MKI49_RS15770 (MKI49_003134) | 59612..60481 | + | 870 | WP_000233000.1 | nucleotidyltransferase domain-containing protein | Toxin |
| MKI49_RS15775 (MKI49_003135) | 60462..61196 | + | 735 | WP_000662263.1 | class I SAM-dependent methyltransferase | - |
| MKI49_RS15780 (MKI49_003136) | 61229..62137 | + | 909 | WP_001255866.1 | aminoglycoside nucleotidyltransferase ANT(6)-Ia | - |
| MKI49_RS15785 (MKI49_003137) | 62134..62614 | + | 481 | Protein_78 | GNAT family N-acetyltransferase | - |
| MKI49_RS15790 (MKI49_003138) | 62707..63501 | + | 795 | WP_001096887.1 | aminoglycoside O-phosphotransferase APH(3')-IIIa | - |
| MKI49_RS16370 (MKI49_003139) | 63911..63994 | + | 84 | Protein_80 | MLS leader peptide | - |
| MKI49_RS15795 (MKI49_003140) | 64043..64126 | + | 84 | WP_033602770.1 | 23S rRNA methyltransferase attenuator leader peptide ErmL | - |
| MKI49_RS15800 (MKI49_003141) | 64251..64988 | + | 738 | WP_001038790.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | ant(6)-Ia / aph(3')-III / erm(B) | cylL / cylS / cylM / cylM / cylM / cylM / cylM / cylM / cylM / cylB / cylB / cylB / cylB / cylB / cylA / cylI / cylI | 1..84898 | 84898 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 290 a.a. Molecular weight: 32926.57 Da Isoelectric Point: 4.8781
>T236691 WP_000233000.1 NZ_CP092578:59612-60481 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
Download Length: 870 bp
>T236691 NZ_LR699570:c756343-755906 [Mycobacterium tuberculosis variant bovis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8365.68 Da Isoelectric Point: 4.5510
>AT236691 WP_000205227.1 NZ_CP092578:59373-59597 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
Download Length: 225 bp
>AT236691 NZ_LR699570:c755796-755551 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|