Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907(toxin) |
| Location | 50445..51016 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP092578 | ||
| Organism | Enterococcus faecalis strain VRE-WC031 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | R3HR30 |
| Locus tag | MKI49_RS15700 | Protein ID | WP_001278770.1 |
| Coordinates | 50445..50786 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A6B1XRC3 |
| Locus tag | MKI49_RS15705 | Protein ID | WP_000958277.1 |
| Coordinates | 50786..51016 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKI49_RS15670 (MKI49_003116) | 46083..46409 | + | 327 | WP_242428005.1 | hypothetical protein | - |
| MKI49_RS15675 (MKI49_003117) | 47296..47571 | - | 276 | WP_000889655.1 | type II toxin-antitoxin system YafQ family toxin | - |
| MKI49_RS15680 (MKI49_003118) | 47564..47830 | - | 267 | WP_000246197.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
| MKI49_RS15685 (MKI49_003119) | 48542..49144 | - | 603 | WP_242427380.1 | Fic family protein | - |
| MKI49_RS15690 | 49427..49672 | - | 246 | WP_242428006.1 | hypothetical protein | - |
| MKI49_RS15695 (MKI49_003120) | 49665..50333 | - | 669 | WP_242428007.1 | hypothetical protein | - |
| MKI49_RS15700 (MKI49_003121) | 50445..50786 | - | 342 | WP_001278770.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| MKI49_RS15705 (MKI49_003122) | 50786..51016 | - | 231 | WP_000958277.1 | hypothetical protein | Antitoxin |
| MKI49_RS15710 (MKI49_003123) | 51246..51851 | + | 606 | WP_000599739.1 | Fic family protein | - |
| MKI49_RS15715 (MKI49_003124) | 51867..52439 | + | 573 | WP_000170424.1 | recombinase family protein | - |
| MKI49_RS15720 (MKI49_003125) | 52872..53831 | - | 960 | WP_000222577.1 | IS30-like element IS1252 family transposase | - |
| MKI49_RS15725 (MKI49_003126) | 53959..54639 | - | 681 | WP_001015311.1 | IS6-like element IS1216 family transposase | - |
| MKI49_RS15730 (MKI49_003127) | 54962..55132 | + | 171 | WP_000713595.1 | hypothetical protein | - |
| MKI49_RS15735 (MKI49_003128) | 55146..55748 | + | 603 | WP_001062586.1 | recombinase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | ant(6)-Ia / aph(3')-III / erm(B) | cylL / cylS / cylM / cylM / cylM / cylM / cylM / cylM / cylM / cylB / cylB / cylB / cylB / cylB / cylA / cylI / cylI | 1..84898 | 84898 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13220.44 Da Isoelectric Point: 8.8595
>T236690 WP_001278770.1 NZ_CP092578:c50786-50445 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T236690 NZ_LR699570:c755564-755256 [Mycobacterium tuberculosis variant bovis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8536.45 Da Isoelectric Point: 4.2412
>AT236690 WP_000958277.1 NZ_CP092578:c51016-50786 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF
Download Length: 231 bp
>AT236690 NZ_LR699570:c755796-755551 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R3HR30 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6B1XRC3 |