Detailed information of TA system TA236689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 24343..24965
• Replicon: plasmid unnamed2
• Accession: NZ_CP092578
• Organism: Enterococcus faecalis strain VRE-WC031

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: MKI49_RS15550
• Protein ID: WP_000241511.1
• Coordinates: 24603..24965 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MKI49_RS15545
• Protein ID: WP_242428033.1
• Coordinates: 24343..24609 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI49_RS15530 (MKI49_003089)	19943..21181	+	1239	WP_002365932.1	S8 family serine peptidase	-
MKI49_RS15535	23188..23511	+	324	WP_242428029.1	hypothetical protein	-
MKI49_RS15540	23546..23800	+	255	WP_242428031.1	hypothetical protein	-
MKI49_RS15545 (MKI49_003091)	24343..24609	+	267	WP_242428033.1	PbsX family transcriptional regulator	Antitoxin
MKI49_RS15550 (MKI49_003092)	24603..24965	+	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI49_RS15555 (MKI49_003093)	25031..25989	-	959	Protein_32	LacI family DNA-binding transcriptional regulator	-
MKI49_RS15560 (MKI49_003094)	25991..27430	-	1440	WP_002383789.1	sucrose-6-phosphate hydrolase	-
MKI49_RS15565 (MKI49_003095)	27621..29552	+	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B)	cylL / cylS / cylM / cylM / cylM / cylM / cylM / cylM / cylM / cylB / cylB / cylB / cylB / cylB / cylA / cylI / cylI	1..84898	84898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (5-88)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T236689 WP_000241511.1 NZ_CP092578:24603-24965 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T236689 NZ_LR699570:c753938-753555 [Mycobacterium tuberculosis variant bovis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10187.63 Da
• Isoelectric Point: 5.7316

>AT236689 WP_242428033.1 NZ_CP092578:24343-24609 [Enterococcus faecalis]
MVMLSIKKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLG
NAVGNEKW

Nucleotide

• Length: 267 bp

>AT236689 NZ_LR699570:c754188-754033 [Mycobacterium tuberculosis variant bovis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: R3H4V2