Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ImmA-IrrE/HTH_19(antitoxin) |
| Location | 2554717..2555415 | Replicon | chromosome |
| Accession | NZ_CP092576 | ||
| Organism | Enterococcus faecalis strain VRE-WC031 | ||
Toxin (Protein)
| Gene name | IrrE | Uniprot ID | A0A059N529 |
| Locus tag | MKI49_RS12790 | Protein ID | WP_002373917.1 |
| Coordinates | 2555071..2555415 (+) | Length | 115 a.a. |
Antitoxin (Protein)
| Gene name | ImmA | Uniprot ID | - |
| Locus tag | MKI49_RS12785 | Protein ID | WP_010782967.1 |
| Coordinates | 2554717..2555052 (+) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKI49_RS12735 (2550237) | 2550237..2551052 | - | 816 | WP_010773519.1 | PD-(D/E)XK nuclease-like domain-containing protein | - |
| MKI49_RS12740 (2551015) | 2551015..2552031 | - | 1017 | WP_002393794.1 | RecT family recombinase | - |
| MKI49_RS12745 (2552134) | 2552134..2552358 | - | 225 | WP_002370012.1 | hypothetical protein | - |
| MKI49_RS12750 (2552355) | 2552355..2552654 | - | 300 | WP_010773520.1 | hypothetical protein | - |
| MKI49_RS12755 (2552741) | 2552741..2553157 | - | 417 | WP_002417286.1 | hypothetical protein | - |
| MKI49_RS12760 (2553158) | 2553158..2553328 | - | 171 | WP_010706857.1 | hypothetical protein | - |
| MKI49_RS12765 (2553363) | 2553363..2553632 | - | 270 | WP_002365131.1 | hypothetical protein | - |
| MKI49_RS12770 (2553650) | 2553650..2553880 | - | 231 | WP_240075834.1 | DUF2829 domain-containing protein | - |
| MKI49_RS12775 (2553892) | 2553892..2554230 | - | 339 | WP_010706859.1 | hypothetical protein | - |
| MKI49_RS12780 (2554243) | 2554243..2554425 | - | 183 | WP_002358110.1 | hypothetical protein | - |
| MKI49_RS12785 (2554717) | 2554717..2555052 | + | 336 | WP_010782967.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| MKI49_RS12790 (2555071) | 2555071..2555415 | + | 345 | WP_002373917.1 | ImmA/IrrE family metallo-endopeptidase | Toxin |
| MKI49_RS12795 (2555440) | 2555440..2555673 | - | 234 | WP_002367290.1 | hypothetical protein | - |
| MKI49_RS12800 (2555685) | 2555685..2556446 | + | 762 | WP_002367291.1 | hypothetical protein | - |
| MKI49_RS12805 (2556622) | 2556622..2557452 | + | 831 | Protein_2503 | site-specific integrase | - |
| MKI49_RS16315 (2557548) | 2557548..2557802 | + | 255 | WP_277956769.1 | tyrosine-type recombinase/integrase | - |
| MKI49_RS12810 (2557905) | 2557905..2558054 | - | 150 | WP_002356321.1 | 50S ribosomal protein L33 | - |
| MKI49_RS12815 (2558180) | 2558180..2560318 | - | 2139 | WP_024041710.1 | penicillin-binding transpeptidase domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 2524228..2557686 | 33458 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 115 a.a. Molecular weight: 13594.61 Da Isoelectric Point: 5.6177
>T236671 WP_002373917.1 NZ_CP092576:2555071-2555415 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMEAEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK
Download Length: 345 bp
>T236671 NZ_LR699119:c1383689-1383462 [Aquicella siphonis]
ATGAAAAAGATGGATAAAGCGACCGCCAATATTATTGATAAGTATTTAACCTTGCGTGTTGCTACCCTTGATGACCCAAC
ACAATTAGGCAAGGCATTGCTACATAATAAAAGCGGTTTATGGCGTTATCGTATAGCCGATCATAGGGTAATTTGCCAGC
TCAACAACAATGAGCTTATCGTGCTGGTATTAAAAGTAGGTCATCGAAAAAATGTTTATGATGAATAA
ATGAAAAAGATGGATAAAGCGACCGCCAATATTATTGATAAGTATTTAACCTTGCGTGTTGCTACCCTTGATGACCCAAC
ACAATTAGGCAAGGCATTGCTACATAATAAAAGCGGTTTATGGCGTTATCGTATAGCCGATCATAGGGTAATTTGCCAGC
TCAACAACAATGAGCTTATCGTGCTGGTATTAAAAGTAGGTCATCGAAAAAATGTTTATGATGAATAA
Antitoxin
Download Length: 112 a.a. Molecular weight: 12828.40 Da Isoelectric Point: 4.8074
>AT236671 WP_010782967.1 NZ_CP092576:2554717-2555052 [Enterococcus faecalis]
MTVFDRIKLLAKERGKSLNRIEEELGLPKNVLYRMKTSDNPTKDRLETLANYFNVSVDYLLGRTDNTKATDEKNSDDLDD
VLDNVMSFDGEPLDDHDREVIRAYLKGRFGK
MTVFDRIKLLAKERGKSLNRIEEELGLPKNVLYRMKTSDNPTKDRLETLANYFNVSVDYLLGRTDNTKATDEKNSDDLDD
VLDNVMSFDGEPLDDHDREVIRAYLKGRFGK
Download Length: 336 bp
>AT236671 NZ_LR699119:c1383930-1383715 [Aquicella siphonis]
ATGGCTATTGCAATTAGGTTATCGCCTGAGCTTGATAAAAGGCTGGATGCGTTGGCTAAAAAAACGCATCGATCTAAAAG
CTATTATGCGCGCGAAGCGATAGAAAATTACATTGAAGATTTGGAAGATTATTATTTGGGCGTGGCTATATTAGAAAACC
CCGGTAATATTTATACGTCTGAAGAAGTGAGGAAAATGTGTGGGCTGGACGATTAA
ATGGCTATTGCAATTAGGTTATCGCCTGAGCTTGATAAAAGGCTGGATGCGTTGGCTAAAAAAACGCATCGATCTAAAAG
CTATTATGCGCGCGAAGCGATAGAAAATTACATTGAAGATTTGGAAGATTATTATTTGGGCGTGGCTATATTAGAAAACC
CCGGTAATATTTATACGTCTGAAGAAGTGAGGAAAATGTGTGGGCTGGACGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|