Detailed information of TA system TA236649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1668295..1668914
• Replicon: chromosome
• Accession: NZ_CP092574
• Organism: Enterococcus faecalis strain VSE-WC032

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: MKI56_RS08425
• Protein ID: WP_000241511.1
• Coordinates: 1668295..1668657 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: MKI56_RS08430
• Protein ID: WP_000245205.1
• Coordinates: 1668651..1668914 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI56_RS08410 (1663705)	1663705..1665636	-	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-
MKI56_RS08415 (1665827)	1665827..1667266	+	1440	WP_002383789.1	sucrose-6-phosphate hydrolase	-
MKI56_RS08420 (1667268)	1667268..1668230	+	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
MKI56_RS08425 (1668295)	1668295..1668657	-	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI56_RS08430 (1668651)	1668651..1668914	-	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
MKI56_RS08435 (1669412)	1669412..1670068	-	657	WP_000411487.1	hypothetical protein	-
MKI56_RS08440 (1672075)	1672075..1672932	-	858	WP_242427385.1	S8 family serine peptidase	-
MKI56_RS08445 (1673147)	1673147..1673314	-	168	WP_242427386.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / aph(3')-III / ant(6)-Ia	cylI / cylA / cylA / cylB / cylB / cylB / cylM / cylM / cylM / cylM / cylM / cylS / cylL / cylR1 / hasC / sprE / gelE / fsrC / fss3	1578709..1961335	382626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T236649 WP_000241511.1 NZ_CP092574:c1668657-1668295 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T236649 NZ_LR699012:c2944219-2943968 [Anaerostipes hadrus]
ATGAATAAATTGTTCACGGATAACGGATGGAAAGACTATGTATATTGGCAAACCGAGGACCGTAAAACCCTAAAAAAAAT
CAATTCACTAATAAATGATATTCTGAGAAATGGAAATGTTGGAATGGGCAAACCAGAACCTTTAACTAGTAATCTATCTG
GCTATTGGAGTCGACGAATTAATGATAAAGATCGTCTTATTTATCGAATTGATGAAGAAAATATTTATATTCTTTCTTGC
AGATATCATTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT236649 WP_000245205.1 NZ_CP092574:c1668914-1668651 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT236649 NZ_LR699012:c2944475-2944212 [Anaerostipes hadrus]
ATGTTAGCAGTAAACTATACAAATTTTCGTGAAAATCTAAAGGATTATATGGATAAAGCAACAGATGATTATGAAACTAT
GATCGTTACACGTAAAAATAATAAAAATGTTGTTATGATTTCTGAAGAATCATACAATAATTTAATGGAAAATGTCTATG
TCATGGGAAACAAATCCAATTATGATTGGTTAATGGAATCTAAAGAACAATTAGAATCTGGAAAATATACAAAGCATGAT
CTTATTGAGGTGCAAGATGAATAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1