Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1642106..1642677 Replicon chromosome
Accession NZ_CP092574
Organism Enterococcus faecalis strain VSE-WC032

Toxin (Protein)


Gene name mazF Uniprot ID R3HR30
Locus tag MKI56_RS08285 Protein ID WP_001278770.1
Coordinates 1642336..1642677 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B1XRC3
Locus tag MKI56_RS08280 Protein ID WP_000958277.1
Coordinates 1642106..1642336 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI56_RS08250 (1637374) 1637374..1637976 - 603 WP_001062586.1 recombinase family protein -
MKI56_RS08255 (1637990) 1637990..1638160 - 171 WP_000713595.1 hypothetical protein -
MKI56_RS08260 (1638483) 1638483..1639163 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
MKI56_RS08265 (1639291) 1639291..1640250 + 960 WP_000222577.1 IS30-like element IS1252 family transposase -
MKI56_RS08270 (1640683) 1640683..1641255 - 573 WP_000170424.1 recombinase family protein -
MKI56_RS08275 (1641271) 1641271..1641876 - 606 WP_000599739.1 Fic family protein -
MKI56_RS08280 (1642106) 1642106..1642336 + 231 WP_000958277.1 hypothetical protein Antitoxin
MKI56_RS08285 (1642336) 1642336..1642677 + 342 WP_001278770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKI56_RS08290 (1642790) 1642790..1643731 + 942 WP_242427379.1 hypothetical protein -
MKI56_RS08295 (1643978) 1643978..1644580 + 603 WP_242427380.1 Fic family protein -
MKI56_RS08300 (1645293) 1645293..1645558 + 266 Protein_1611 type II toxin-antitoxin system RelB/DinJ family antitoxin -
MKI56_RS08305 (1645551) 1645551..1645826 + 276 WP_000889655.1 type II toxin-antitoxin system YafQ family toxin -
MKI56_RS08310 (1646737) 1646737..1646997 - 261 WP_242427381.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) / aph(3')-III / ant(6)-Ia cylI / cylA / cylA / cylB / cylB / cylB / cylM / cylM / cylM / cylM / cylM / cylS / cylL / cylR1 / hasC / sprE / gelE / fsrC / fss3 1578709..1961335 382626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-112)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 13220.44 Da        Isoelectric Point: 8.8595

>T236648 WP_001278770.1 NZ_CP092574:1642336-1642677 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T236648 NZ_LR699011:3318395-3318685 [Roseburia hominis]
ATGAGGAAAACAAAGTACACCGTCAAGTACACGACCGCTTTCAAAAAGGACTACAAGCGGGCAATCAAGCGCGGCCTGAA
AATCGAATTGCTGGAACAGGTCGTGGCGCTGCTGTCGATGGGCGAACCGCTGCCGGATAAAAACCGCGATCACGATTTGT
CCGGCGATTGGGCAGGCCATCGAGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGAGGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.45 Da        Isoelectric Point: 4.2412

>AT236648 WP_000958277.1 NZ_CP092574:1642106-1642336 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Download         Length: 231 bp

>AT236648 NZ_LR699011:3318120-3318398 [Roseburia hominis]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAGCT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAGGAAACGATTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGATAC
AATGACCTTGACGAGTTGTTTGCCGACCTGAAAAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB R3HR30


Antitoxin

Source ID Structure
AlphaFold DB A0A6B1XRC3

References