Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Xre-MNT/HTH_26(antitoxin) |
| Location | 1632641..1633749 | Replicon | chromosome |
| Accession | NZ_CP092574 | ||
| Organism | Enterococcus faecalis strain VSE-WC032 | ||
Toxin (Protein)
| Gene name | MNTss | Uniprot ID | R3JIN1 |
| Locus tag | MKI56_RS08220 | Protein ID | WP_000233000.1 |
| Coordinates | 1632641..1633510 (-) | Length | 290 a.a. |
Antitoxin (Protein)
| Gene name | Xress | Uniprot ID | - |
| Locus tag | MKI56_RS08225 | Protein ID | WP_000205227.1 |
| Coordinates | 1633525..1633749 (-) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKI56_RS08190 (1628134) | 1628134..1628871 | - | 738 | WP_001038790.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| MKI56_RS08195 (1628996) | 1628996..1629079 | - | 84 | WP_033602770.1 | 23S rRNA methyltransferase attenuator leader peptide ErmL | - |
| MKI56_RS15470 (1629128) | 1629128..1629211 | - | 84 | Protein_1590 | MLS leader peptide | - |
| MKI56_RS08200 (1629621) | 1629621..1630415 | - | 795 | WP_001096887.1 | aminoglycoside O-phosphotransferase APH(3')-IIIa | - |
| MKI56_RS08205 (1630508) | 1630508..1630988 | - | 481 | Protein_1592 | GNAT family N-acetyltransferase | - |
| MKI56_RS08210 (1630985) | 1630985..1631893 | - | 909 | WP_001255866.1 | aminoglycoside nucleotidyltransferase ANT(6)-Ia | - |
| MKI56_RS08215 (1631926) | 1631926..1632660 | - | 735 | WP_000662263.1 | class I SAM-dependent methyltransferase | - |
| MKI56_RS08220 (1632641) | 1632641..1633510 | - | 870 | WP_000233000.1 | nucleotidyltransferase domain-containing protein | Toxin |
| MKI56_RS08225 (1633525) | 1633525..1633749 | - | 225 | WP_000205227.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| MKI56_RS08230 (1633892) | 1633892..1635436 | - | 1545 | WP_002390960.1 | recombinase family protein | - |
| MKI56_RS08235 (1635456) | 1635456..1635872 | - | 417 | WP_000323438.1 | recombinase | - |
| MKI56_RS08240 (1635873) | 1635873..1636199 | - | 327 | WP_000392725.1 | hypothetical protein | - |
| MKI56_RS08245 (1636681) | 1636681..1637358 | - | 678 | Protein_1600 | DNA topoisomerase | - |
| MKI56_RS08250 (1637374) | 1637374..1637976 | - | 603 | WP_001062586.1 | recombinase family protein | - |
| MKI56_RS08255 (1637990) | 1637990..1638160 | - | 171 | WP_000713595.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | erm(B) / aph(3')-III / ant(6)-Ia | cylI / cylA / cylA / cylB / cylB / cylB / cylM / cylM / cylM / cylM / cylM / cylS / cylL / cylR1 / hasC / sprE / gelE / fsrC / fss3 | 1578709..1961335 | 382626 | |
| - | flank | IS/Tn | erm(B) / aph(3')-III / ant(6)-Ia | - | 1627149..1631893 | 4744 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 290 a.a. Molecular weight: 32926.57 Da Isoelectric Point: 4.8781
>T236647 WP_000233000.1 NZ_CP092574:c1633510-1632641 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI
Download Length: 870 bp
>T236647 NZ_LR699011:2265641-2265931 [Roseburia hominis]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAGGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATTGAGCTGCTGGAGCAGGTCGTAGCATTGCTGGCAATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGTCATCGGGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTATCGCATAGAGGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAGGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATTGAGCTGCTGGAGCAGGTCGTAGCATTGCTGGCAATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGTCATCGGGAATGTCATATTCTCCCGGACTGGCTGCTTGTCTATCGCATAGAGGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8365.68 Da Isoelectric Point: 4.5510
>AT236647 WP_000205227.1 NZ_CP092574:c1633749-1633525 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE
Download Length: 225 bp
>AT236647 NZ_LR699011:2265366-2265644 [Roseburia hominis]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACGGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACGGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|