Detailed information of TA system TA236642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 76332..76851
• Replicon: plasmid unnamed2
• Accession: NZ_CP092571
• Organism: Enterococcus faecium strain VRE-WC072

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: MKI62_RS15120
• Protein ID: WP_000588503.1
• Coordinates: 76594..76851 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: MKI62_RS15115
• Protein ID: WP_000388479.1
• Coordinates: 76332..76601 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI62_RS15080 (MKI62_003010)	71903..72319	-	417	WP_001232859.1	hypothetical protein	-
MKI62_RS15085 (MKI62_003011)	72322..72606	-	285	WP_000353844.1	hypothetical protein	-
MKI62_RS15090 (MKI62_003012)	72972..73196	+	225	WP_000410576.1	hypothetical protein	-
MKI62_RS15095 (MKI62_003014)	74319..74999	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
MKI62_RS15100 (MKI62_003015)	75075..75641	+	567	Protein_90	hypothetical protein	-
MKI62_RS15105	75806..76006	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
MKI62_RS15110 (MKI62_003016)	76022..76201	+	180	WP_001120991.1	hypothetical protein	-
MKI62_RS15115 (MKI62_003017)	76332..76601	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI62_RS15120 (MKI62_003018)	76594..76851	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
MKI62_RS15125 (MKI62_003019)	77489..78313	+	825	WP_001809248.1	hypothetical protein	-
MKI62_RS15130 (MKI62_003020)	78478..79092	-	615	WP_001261742.1	recombinase family protein	-
MKI62_RS15135 (MKI62_003021)	79542..80867	+	1326	WP_000969590.1	Y-family DNA polymerase	-
MKI62_RS15140 (MKI62_003022)	80860..81210	+	351	WP_000568378.1	hypothetical protein	-
MKI62_RS15145 (MKI62_003023)	81207..81386	+	180	WP_001021554.1	hypothetical protein	-
MKI62_RS15150 (MKI62_003024)	81522..81812	+	291	WP_000026576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / tet(S) / VanHAX	-	1..83629	83629

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T236642 WP_000588503.1 NZ_CP092571:76594-76851 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T236642 NZ_LR699009:5250364-5250639 [Pluralibacter gergoviae]
ATGAGGTTTCAGTGGAGCGTACCAGCCAGAAACGATGTTGTCCGTCTGCACCAGTTTCTGGCCGATAAGAATGAGGCCGC
AACAGCAAATGTGGTCAGATCTCTGATTAACGCTCCCGCCACCCTACTGCTCAACCCTCGACGGGGAGAGAAAATAGAGG
GGTTTGAACCTCGCGAAGTGCGGCGCGCCATCATCGGCAGCTATGAAATGCATTATGAGCTGCAGAATGATGTATTAATT
GTTCTGCGCATCTGGCACCAGCGCGAGGATCGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT236642 WP_000388479.1 NZ_CP092571:76332-76601 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT236642 NZ_LR699009:5250095-5250367 [Pluralibacter gergoviae]
ATGGCTGATATGCGTACTATCACAGCTCCCCTGCCGATTGAGATGGCTGAAAGCGTCGATCGCTGGGCTGAAAAGCACGG
CCGCACCCGCTCATGGTTAGTCAGAGAAGCCGTTGGCCAATTTCTTGCGCGTGAAAATGAGCGCGATCGGCTGATACGCG
AGGCGCTTGACGCCGTCGATGCCGGACAGCTTATTTCTCATGAAGATATGGTCGCCTGGGCCGAATCACTCGGCACAGAT
AAGCCGCTCCCACCGCCGGAGCCGCGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7