Detailed information of TA system TA236633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2144222..2144751
• Replicon: chromosome
• Accession: NZ_CP092567
• Organism: Staphylococcus aureus strain MRSA-WC000

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI50_RS10760
• Protein ID: WP_000621175.1
• Coordinates: 2144222..2144584 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI50_RS10765
• Protein ID: WP_000948331.1
• Coordinates: 2144581..2144751 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI50_RS10740 (MKI50_002127)	2141200..2141970	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI50_RS10745 (MKI50_002128)	2141945..2142424	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI50_RS10750 (MKI50_002129)	2142426..2142752	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI50_RS10755 (MKI50_002130)	2142871..2143872	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI50_RS10760 (MKI50_002131)	2144222..2144584	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI50_RS10765 (MKI50_002132)	2144581..2144751	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI50_RS10770 (MKI50_002133)	2144836..2145984	-	1149	WP_001281154.1	alanine racemase	-
MKI50_RS10775 (MKI50_002134)	2146050..2146409	-	360	WP_000581197.1	holo-ACP synthase	-
MKI50_RS10780 (MKI50_002135)	2146413..2146904	-	492	WP_001205912.1	PH domain-containing protein	-
MKI50_RS10785 (MKI50_002136)	2146891..2148474	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI50_RS10790 (MKI50_002137)	2148467..2148946	-	480	WP_001287087.1	hypothetical protein	-
MKI50_RS10795 (MKI50_002138)	2149154..2149714	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236633 WP_000621175.1 NZ_CP092567:c2144584-2144222 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236633 NZ_LR699009:c1922403-1922005 [Pluralibacter gergoviae]
GTGAGGGTTTTTCGAACTAAGCTATTCAATAAGGGAGCAAAAAATTTCCAGCTCCCTGACACAGCATTATTCGCCGTTGC
CAGGGAGGTCTTTGAAGGTCGGTATGAAGCCAATCTCGGCGGTGGCGTGATTAAGAAACGCATAGCGATCAACGGGAAAG
GAAAGCGCGGAGGTATTCGCAGCATCATCTTTTTTAAAGTTGGCAGCCATATTTTCTTCGCCGACACGTGGAGTAAAAAC
GATACGCCCAAGAGCGGTAAAGAGATTGATGATGCACAACTCTATGCGTATCGATTGCTGGCAGAAACATTGCTTAATCT
GAACGACAAACAGTTAATAGAGATGATTGCAAAGAACCTGCTCACTGAAGTCAAAGAATGTGAGGATACTGATGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236633 WP_000948331.1 NZ_CP092567:c2144751-2144581 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236633 NZ_LR699009:c1922012-1921683 [Pluralibacter gergoviae]
ATGAGTGATAAAATGACCGATATTCTTGAGTTCTCAAAAGAGCTCAATAACGCCGGGATCCTGAGCGATGATAAGTACGC
GAAAATTAATGCCCGAGCCAAAGCGTGTAAAATAACGGCGCAAATCCAGCCTCTCAGGCCGATGAGCGGTGAAGAAATAA
GGACAATGCGCAGCCACTACGGCATGTCCCAAACAATGCTGGCCGCCACCCTTGGCATAACGACCGCAAGCGTTTCGAAA
TGGGAACGTGATGTTGTACAACCCAGCGGCCCGGCGCTGCGCTTATTGAACACGCTGCAGCTTAAAGGGCCCGATATTTT
TATCGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1