Detailed information of TA system TA236631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1931301..1932077
• Replicon: chromosome
• Accession: NZ_CP092567
• Organism: Staphylococcus aureus strain MRSA-WC000

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: MKI50_RS09400
• Protein ID: WP_000031108.1
• Coordinates: 1931301..1931453 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: MKI50_RS09405
• Protein ID: WP_001251224.1
• Coordinates: 1931478..1932077 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI50_RS09385 (MKI50_001859)	1927503..1928324	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
MKI50_RS09390 (MKI50_001860)	1928777..1930162	-	1386	WP_000116228.1	class II fumarate hydratase	-
MKI50_RS09395 (MKI50_001861)	1930358..1930753	-	396	WP_000901023.1	hypothetical protein	-
MKI50_RS09400 (MKI50_001862)	1931301..1931453	-	153	WP_000031108.1	SAS053 family protein	Toxin
MKI50_RS09405 (MKI50_001863)	1931478..1932077	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
MKI50_RS09410 (MKI50_001864)	1932236..1932706	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
MKI50_RS09415 (MKI50_001865)	1932711..1933838	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
MKI50_RS09420 (MKI50_001866)	1933989..1934711	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
MKI50_RS09425 (MKI50_001867)	1934704..1936161	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T236631 WP_000031108.1 NZ_CP092567:c1931453-1931301 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T236631 NZ_LR699009:1328996-1329337 [Pluralibacter gergoviae]
ATGGATGCGCTGTTTATTGAACTACCGCCCTTTGAACGGCATCGTGCTGAATACTTCAGCGATGATGATTTCAAGGCTTT
CCAGCAAATGTTGTTGCATAATCCTTGCGCCGGGGATGTCATTCGAGGCGCCGGGGGACTTCGTAAAATTCGATTTGCTG
ACCCTCGCAGGAATAAGGGGAAGCGGGGAGGTATTCGGGTTATCTATTACTGGTATGCCGAGAAGTCCCATTTTCTGCTT
TTTACGCTATACGATAAAGAGCAGCAGGATGATTTGACGGCTCACCAACGTAACATCCTGTCCCATCTTTTAGAGCAGGC
AAAACAGAGGATCATCAGGTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT236631 WP_001251224.1 NZ_CP092567:c1932077-1931478 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT236631 NZ_LR699009:1329334-1329645 [Pluralibacter gergoviae]
GTGACGAAGCGAGATATTTTCAGCGAATTGCAGGAAGGTATTGAAGCGTGGGGCGAGTTAAATGCCGGAAAAAAAACACT
GCGCACTCACCGCGTTAATACCCGCGATCTGGCGATTGCGCCGGAAGATTTGGTCAAGGTTCGTGAACAGCTCAATCTTT
CGCAGGCCGTTTTCGCGCGCTATCTTCATGCGGGCCTGAAGACTTACCAGAATTGGGAGCAGGGCCTTGCCAGTCCAAAC
AAACAGGCGGTGCTATTGATCCGCATGATTGAGAAAAGTCCCTCTGTATTGTCTCAGCTTGCCGCTATCTAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4