Detailed information of TA system TA236625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 848339..848819
• Replicon: chromosome
• Accession: NZ_CP092567
• Organism: Staphylococcus aureus strain MRSA-WC000

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: -
• Locus tag: MKI50_RS04080
• Protein ID: WP_242417786.1
• Coordinates: 848339..848530 (-)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MKI50_RS04085
• Protein ID: WP_001059690.1
• Coordinates: 848559..848819 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI50_RS04050 (MKI50_000796)	844333..844854	+	522	WP_000824482.1	hypothetical protein	-
MKI50_RS04055 (MKI50_000797)	845195..845881	+	687	WP_000141557.1	thermonuclease family protein	-
MKI50_RS04060 (MKI50_000798)	846238..846438	+	201	WP_001059082.1	cold-shock protein	-
MKI50_RS04065 (MKI50_000799)	846930..847148	-	219	WP_000005886.1	hypothetical protein	-
MKI50_RS04070 (MKI50_000800)	847210..847494	-	285	WP_001004399.1	hypothetical protein	-
MKI50_RS04075 (MKI50_000801)	847582..848151	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
MKI50_RS04080 (MKI50_000802)	848339..848530	-	192	WP_242417786.1	hypothetical protein	Toxin
MKI50_RS04085 (MKI50_000803)	848559..848819	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MKI50_RS04090 (MKI50_000804)	849162..849365	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MKI50_RS04095 (MKI50_000805)	849362..849598	-	237	WP_000349581.1	hypothetical protein	-
MKI50_RS04100 (MKI50_000806)	849823..850404	+	582	WP_001140693.1	histidine phosphatase family protein	-
MKI50_RS04105 (MKI50_000807)	850477..851094	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
MKI50_RS04110 (MKI50_000808)	851249..851743	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
MKI50_RS04115 (MKI50_000809)	852142..852564	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
MKI50_RS04120 (MKI50_000810)	852712..853428	+	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-138)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7289.99 Da
• Isoelectric Point: 9.9971

>T236625 WP_242417786.1 NZ_CP092567:c848530-848339 [Staphylococcus aureus]
MSLHFAILFWLALIFFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 192 bp

>T236625 NZ_LR699008:3825783-3826094 [Hafnia alvei]
ATGCACATCATATCTAGGGATCCGTTTGAAGACGCCGCGCGAAAATACCCAAACGATGCCGCATCTCTCATAGCAGCATA
CCGATTGCTTAAAGAGAATGAATTTGAAAGTCCTGCAGAATTACGCAAACTGTTTCCTAACCTCGATAACTTCAAATATA
GGAACAAGTGGTGGGTAATCAACATTGGTGGCAACAATCTGCGGATGATTTCCTACATCAACTTTGTAAACCAGCGCTTT
TACGTCAAACACATCGTCACCCACGCAGAGTATGACAAGCTAACGAGGCAGTACCGGGAGATAAAAGAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT236625 WP_001059690.1 NZ_CP092567:c848819-848559 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT236625 NZ_LR699008:3826091-3826510 [Hafnia alvei]
ATGATTTTAGAACCGACCAAAGCGCTAGAGGCCGCAAAAGTATTGGCAGATGCCGTGCCTTTTTTAGGCGGCAATACTTC
TGAGGAGGCCTATCGCGATGCGCTCGCTATGGTTGATTATCTTATCGAGCACGATGACGAAAGCCCGCTAATAGATTTTC
TAGCGTTAAAAATTGCTGAATATGAAGATAACAATGAGCGATTTAAAGCCTTCAATCAGGCCGTGACTGATATGCCTACT
GGCGTGGCCGCGCTACGGGTTCTTATCGAACAACATCAGCTTTCCTACTCAGATTTAGCCTCAGAAATTGGTTCAAAAAG
CCTCGTCAGCATGATTTTATCCGGCAAACGCTCATTAACCATTGAGCATATCAAAGCACTCTCAAAGCGCTTTAACGTTA
GACCAGACCTGTTTTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VN56