Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2498943..2499466 Replicon chromosome
Accession NZ_CP092565
Organism Staphylococcus aureus strain MRSA-WC061

Toxin (Protein)


Gene name relE Uniprot ID A0A3A5LQI3
Locus tag MKI65_RS12490 Protein ID WP_000074049.1
Coordinates 2498943..2499209 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID W8TQT5
Locus tag MKI65_RS12495 Protein ID WP_000587616.1
Coordinates 2499209..2499466 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI65_RS12470 (MKI65_002471) 2494250..2495437 - 1188 WP_000026189.1 MFS transporter -
MKI65_RS12475 (MKI65_002472) 2495833..2496609 - 777 WP_000072149.1 iron export ABC transporter permease subunit FetB -
MKI65_RS12480 (MKI65_002473) 2496602..2497264 - 663 WP_000923526.1 ATP-binding cassette domain-containing protein -
MKI65_RS12485 (MKI65_002474) 2497512..2498588 - 1077 WP_001076673.1 M42 family metallopeptidase -
MKI65_RS12490 (MKI65_002475) 2498943..2499209 - 267 WP_000074049.1 Txe/YoeB family addiction module toxin Toxin
MKI65_RS12495 (MKI65_002476) 2499209..2499466 - 258 WP_000587616.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MKI65_RS12500 (MKI65_002477) 2499825..2500280 + 456 WP_000732076.1 DUF1307 domain-containing protein -
MKI65_RS12505 (MKI65_002478) 2500448..2502013 - 1566 WP_000143492.1 FMN-binding glutamate synthase family protein -
MKI65_RS12510 (MKI65_002479) 2502208..2502957 + 750 WP_000072461.1 CPBP family lipoprotein N-acylation protein LnsB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(4-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10676.24 Da        Isoelectric Point: 10.1597

>T236624 WP_000074049.1 NZ_CP092565:c2499209-2498943 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLRKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Download         Length: 267 bp

>T236624 NZ_LR699008:3227929-3228132 [Hafnia alvei]
ATGACTAAAATTGATTACCTTATGCGCTTACGAAAGTGTACAACGTTAGAAACTTTAGAGCGCGTAATTGAAAAAAACAA
ATATGAGCTATCCGACGATGAGCTAGAAACTTTCTACTCAGCCGCCGACCATCGTTTGGCCGAACTCACCATGAACAAAC
TGTATGATAAGATCCCTGCTGAAGTTTGGAAGTATGTTCGCTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9774.73 Da        Isoelectric Point: 4.1353

>AT236624 WP_000587616.1 NZ_CP092565:c2499466-2499209 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Download         Length: 258 bp

>AT236624 NZ_LR699008:3227541-3227909 [Hafnia alvei]
ATGGATGAGTACACGTCTTATCAGCACGATATAACTGAACTGAAATACCTGTGTAATATGCTGTATAATCAGGGAATGGA
AGTTCTGAGTGATAGCCATCATGGCTGGGTTAATGACCCAACTGCTGTAGCTAATCTGCAACTCAATGAATTAAACGAAC
ATATCGCAACGTTCGGTTTAACGTTCAAGATTAAGTATCCAAAGAGTTCAGAATTAACTGAAATATTAGATGAGTATCTT
GACGAAACTTACAGTCTGTTTAGTAATTACAGCATAAACGAACCAGAACTAAAAAAATGGCTTAAAGTAAAAGGCCGTAT
TTTGCGTTATTTAGCGGGTGAAAAACCAGCATCCGCGTTGAACTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A5LQI3

Antitoxin

Source ID Structure
PDB 7V6W
PDB 7V5Z
PDB 7V5Y
AlphaFold DB W8TQT5

References