Detailed information of TA system TA023661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1031078..1032315
• Replicon: chromosome
• Accession: NC_012468
• Organism: Streptococcus pneumoniae 70585

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: C1C756
• Locus tag: SP70585_RS05610
• Protein ID: WP_000405355.1
• Coordinates: 1031554..1032315 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: SP70585_RS05605
• Protein ID: WP_000579612.1
• Coordinates: 1031078..1031554 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SP70585_RS05580	1026151..1027005	+	855	WP_000901912.1	ADP-ribosylglycohydrolase family protein	-
SP70585_RS05585	1027117..1028001	+	885	WP_000757475.1	diacylglycerol kinase family lipid kinase	-
SP70585_RS12465	1028140..1028241	+	102	WP_096376117.1	alpha amylase N-terminal ig-like domain-containing protein	-
SP70585_RS05590	1028305..1029903	+	1599	WP_012677142.1	glycoside hydrolase family 13 protein	-
SP70585_RS05600	1030178..1030633	+	456	WP_000500472.1	hypothetical protein	-
SP70585_RS05605	1031078..1031554	+	477	WP_000579612.1	helix-turn-helix domain-containing protein	Antitoxin
SP70585_RS05610	1031554..1032315	+	762	WP_000405355.1	zeta toxin family protein	Toxin
SP70585_RS05615	1032343..1033500	+	1158	WP_001213950.1	hypothetical protein	-
SP70585_RS05625	1034332..1034691	+	360	WP_000529544.1	hypothetical protein	-
SP70585_RS05630	1034701..1035066	+	366	WP_000263052.1	MobC family plasmid mobilization relaxosome protein	-
SP70585_RS12470	1035053..1036887	+	1835	Protein_1063	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	970520..1052224	81704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 28960.87 Da
• Isoelectric Point: 6.6165

>T23661 WP_000405355.1 NC_012468:1031554-1032315 [Streptococcus pneumoniae 70585]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLGYNLLIEGTLRTIDVPKETAQLLKSKGYEVQLALIATKSELSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAAEVLQEYLFGKWSKVEEEML
KVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T23661 NC_012468:1031554-1032315 [Streptococcus pneumoniae 70585]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTCTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCTGGTAAGACTACAATTCATCGAATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGCGTTGAATACACCAAAGATTTTGCAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGGGATACAATCTTTTGATCGAGGGAACTTTACGAACGATTGACGTTCCAAAGGAAACAGCACAACTCTTGAAAAGTA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAGTCTGAGCTGTCCTATCTGAGCACTCTTATCCGATACGAAGAA
CTGTACGCTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTGGCTATCTTTGAAAGCATTCAAATTTATCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGAAGTGCTACAAGAATACCTCTTCGGAAAATGGAGCAAGGTAGAGGAAGAGATGTTG
AAGGTGGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT23661 WP_000579612.1 NC_012468:1031078-1031554 [Streptococcus pneumoniae 70585]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT23661 NC_012468:1031078-1031554 [Streptococcus pneumoniae 70585]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTACTTTCTCGACTCTATCGCTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGATTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: C1C756

Antitoxin

• AlphaFold DB: B6ZCC4