Detailed information of TA system TA236608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2102701..2103230
• Replicon: chromosome
• Accession: NZ_CP092563
• Organism: Staphylococcus aureus strain MRSA-WC090

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI59_RS10430
• Protein ID: WP_000621175.1
• Coordinates: 2102701..2103063 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI59_RS10435
• Protein ID: WP_000948331.1
• Coordinates: 2103060..2103230 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI59_RS10410 (MKI59_002066)	2099679..2100449	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI59_RS10415 (MKI59_002067)	2100424..2100903	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI59_RS10420 (MKI59_002068)	2100905..2101231	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI59_RS10425 (MKI59_002069)	2101350..2102351	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI59_RS10430 (MKI59_002070)	2102701..2103063	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI59_RS10435 (MKI59_002071)	2103060..2103230	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI59_RS10440 (MKI59_002072)	2103315..2104463	-	1149	WP_001281154.1	alanine racemase	-
MKI59_RS10445 (MKI59_002073)	2104529..2104888	-	360	WP_000581197.1	holo-ACP synthase	-
MKI59_RS10450 (MKI59_002074)	2104892..2105383	-	492	WP_001205912.1	PH domain-containing protein	-
MKI59_RS10455 (MKI59_002075)	2105370..2106953	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI59_RS10460 (MKI59_002076)	2106946..2107425	-	480	WP_001287087.1	hypothetical protein	-
MKI59_RS10465 (MKI59_002077)	2107633..2108193	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236608 WP_000621175.1 NZ_CP092563:c2103063-2102701 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236608 NZ_LR699006:c957500-957189 [Citrobacter freundii]
ATGCTATTCATAGAAACGGAAATCTTTACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGACTACAGAT
ATTCCTTACGATTCAGCCCGATTGTGGCGATCTGATACATGATACAGGGGGCCTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGAAAACGTGGCGGTGTAAGGATTATCTACTTTCATCAAATCAGGAAATCACAAATTCGTTTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAGGAAAAAGTACTGCTGCGTATGTTGAATGAGGGGTGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236608 WP_000948331.1 NZ_CP092563:c2103230-2103060 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236608 NZ_LR699006:c957188-956901 [Citrobacter freundii]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGATTCAGATGAATGAGATCGATGAAGGAACGCGGCAGCCTTC
TCGTACCTTTCATATTGACGCCATGAAAATAAAGGAAATCCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGAAATTGGGAGCAGGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCCATTGCCAATGATCCAAAACATGTCATTCAGGCGCTAAGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1