Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 1889786..1890562 Replicon chromosome
Accession NZ_CP092563
Organism Staphylococcus aureus strain MRSA-WC090

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag MKI59_RS09070 Protein ID WP_000031108.1
Coordinates 1889786..1889938 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID W8U4V4
Locus tag MKI59_RS09075 Protein ID WP_001251224.1
Coordinates 1889963..1890562 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI59_RS09055 (MKI59_001798) 1885988..1886809 + 822 WP_000669376.1 RluA family pseudouridine synthase -
MKI59_RS09060 (MKI59_001799) 1887262..1888647 - 1386 WP_000116228.1 class II fumarate hydratase -
MKI59_RS09065 (MKI59_001800) 1888843..1889238 - 396 WP_000901023.1 hypothetical protein -
MKI59_RS09070 (MKI59_001801) 1889786..1889938 - 153 WP_000031108.1 SAS053 family protein Toxin
MKI59_RS09075 (MKI59_001802) 1889963..1890562 - 600 WP_001251224.1 glucosamine-6-phosphate isomerase Antitoxin
MKI59_RS09080 (MKI59_001803) 1890721..1891191 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
MKI59_RS09085 (MKI59_001804) 1891196..1892323 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
MKI59_RS09090 (MKI59_001805) 1892474..1893196 - 723 WP_000590809.1 amino acid ABC transporter ATP-binding protein -
MKI59_RS09095 (MKI59_001806) 1893189..1894646 - 1458 WP_000649908.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T236606 WP_000031108.1 NZ_CP092563:c1889938-1889786 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T236606 NZ_LR699006:c399282-398893 [Citrobacter freundii]
ATGCAAACCAAATCATCCCAACTCATGCGGGAGGCATTGTCACGTCCGTCTGCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTTCCTGCTGGAACACCACTATGGTCTCACGCTTAATGACACGCCGTTCAGTCATGAGCAGGTGATTCAGCAGCATATCG
ACGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTCGAAAAGTATGAACTGCTTCGCACTGACCGGCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCGAGG
CACGTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAGGACTTCAGGCAAGCGATGA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22343.47 Da        Isoelectric Point: 5.1445

>AT236606 WP_001251224.1 NZ_CP092563:c1890562-1889963 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT236606 NZ_LR699006:c399664-399338 [Citrobacter freundii]
ATCAGCGCCCCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGGGCACGTCTGGTCCAGGAGGGAAATCGCCT
GCATTACCTGGCTGACCGGGCAAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TACTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATTCCCTCCACCAGCACTGCGTCACGTTATATGCT
AAAGGGCTGACCTGTGAGGCCGATACGCTCGGTTCGCATGGTTACGTTTATATCGCGATTTATCCCACTCCTGCCGTCAC
GGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB W8U4V4

References