Detailed information of TA system TA236597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2101816..2102345
• Replicon: chromosome
• Accession: NZ_CP092561
• Organism: Staphylococcus aureus strain MRSA-WC101

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI54_RS10435
• Protein ID: WP_000621175.1
• Coordinates: 2101816..2102178 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI54_RS10440
• Protein ID: WP_000948331.1
• Coordinates: 2102175..2102345 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI54_RS10415 (MKI54_002065)	2098794..2099564	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI54_RS10420 (MKI54_002066)	2099539..2100018	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI54_RS10425 (MKI54_002067)	2100020..2100346	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI54_RS10430 (MKI54_002068)	2100465..2101466	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI54_RS10435 (MKI54_002069)	2101816..2102178	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI54_RS10440 (MKI54_002070)	2102175..2102345	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI54_RS10445 (MKI54_002071)	2102430..2103578	-	1149	WP_001281154.1	alanine racemase	-
MKI54_RS10450 (MKI54_002072)	2103644..2104003	-	360	WP_000581197.1	holo-ACP synthase	-
MKI54_RS10455 (MKI54_002073)	2104007..2104498	-	492	WP_001205912.1	PH domain-containing protein	-
MKI54_RS10460 (MKI54_002074)	2104485..2106068	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI54_RS10465 (MKI54_002075)	2106061..2106540	-	480	WP_001287087.1	hypothetical protein	-
MKI54_RS10470 (MKI54_002076)	2106748..2107308	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236597 WP_000621175.1 NZ_CP092561:c2102178-2101816 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236597 NZ_LR698987:3382537-3383469 [Leminorella richardii]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTCAGGCTATTAATGGCTGCCCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCATTGAAAGGCGGCACAGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATCGATCTGGCGTATA
AGACCTTTGCGGATCGCGATACCGATTTGGCAGCTATCAACGATGCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATCACCGCGATTCGACAGGAAAATAAAGCGGATGAGAAGCGCATCATTGTGAATACCACTGATGCACAGATAAA
GATCGAGGTTAGTCCAGTCTGGCGCGGGCTGCTGCTGCCACCAACAGAAATGCCAGTATGTGAACAGGTGGAAATGGAAT
ATGGATTCACCACCATGAATGTCGTTTCGCTGGCAGATCTTTATGGCGGTAAAATCTGCGCCGCGCTCGATCGCCAGCAT
CCGCGCGATCTGTTTGACGTGCTAAATATGCTGAAAAAACCGGGCCTGACGCGCGAGATCTTTGACGGTTTTCTTTGCTA
TCTGGCAGGCCATCCTCGCCCCATTGCTGAGTTACTGACTCCCAACTGGGATACAGCGCGGATCGCCACATTGTACGCAC
AGGAATTTTCAGGAATGACAACACAGCGGGAAACATCGCTGGAGTCGCTTTTATCAGTAACGACGTTGTTGCCACAAGCA
CTGAAATCGCATCTTACGGACAGGGATCGCCAGTTCTTACTCAGTTATAAGCAAAATACCCCTGACTGGTCGCTGTATCG
CTATCCTGAAATTCAACACCTACCGGCTATTCGCTGGAAGCTGCGTAATCTGGCCGTACTGAAGGAAAAGAACCCGGCCA
AGAATACGGCGGCGGTGAGCAAGTTAGAAGGAGTTCTGGAGCAGTACTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236597 WP_000948331.1 NZ_CP092561:c2102345-2102175 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236597 NZ_LR698987:3381768-3382550 [Leminorella richardii]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACCCTCCCTGGTCAGGTGCTGCTCCAATCATGGCTTACCCGTAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTCCAGAATGGCTGGCTCAGACGAATCGCTCACGGCGTTTACTGCCGGACGG
GTCGCGATCCAGACTGGGTGGATGCGGTTTACTGCCTGCAAACGCAGTGGGAAAAACCGGTTCGTGTAGCGGGGCTGACC
AGCCTTGCACTACAGGGCCACGCTCACTATACCGAGCCGGGCAGACAACAAATCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTCGCCACCTTTGAACAATCCGCGACGTTTGTCACGCTGTATACGTCAGGCCTTACCGTTGATATCA
CGTTATTGACCACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGTCCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGTGTTCCTTCGTTGATCTCGTTCGAGCATGCCGATGCTCTGTTTCAGGGAATGAATCTTCTCAGTCCGCGTAAATT
GCAAACACTGCTCTGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTCTATCTTGCCCGGCGCAATGAGCATCCTT
ATTTTCGGTATCTCGATCAAAGCGGTATAGAAACAGGTACGGGAAAACGGCAAATCGTTCCCGGCGGCTGGCTGGAACCG
GATTATCAAATCACCGTTCCCCGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1