Detailed information of TA system TA236592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 851388..851865
• Replicon: chromosome
• Accession: NZ_CP092561
• Organism: Staphylococcus aureus strain MRSA-WC101

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: MKI54_RS04090
• Protein ID: WP_000052076.1
• Coordinates: 851388..851576 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MKI54_RS04095
• Protein ID: WP_001059690.1
• Coordinates: 851605..851865 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI54_RS04060 (MKI54_000801)	847382..847903	+	522	WP_000824482.1	hypothetical protein	-
MKI54_RS04065 (MKI54_000802)	848244..848930	+	687	WP_000141557.1	thermonuclease family protein	-
MKI54_RS04070 (MKI54_000803)	849287..849487	+	201	WP_001059082.1	cold-shock protein	-
MKI54_RS04075 (MKI54_000804)	849979..850197	-	219	WP_000005886.1	hypothetical protein	-
MKI54_RS04080 (MKI54_000805)	850259..850543	-	285	WP_001004399.1	hypothetical protein	-
MKI54_RS04085 (MKI54_000806)	850631..851200	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
MKI54_RS04090 (MKI54_000807)	851388..851576	-	189	WP_000052076.1	hypothetical protein	Toxin
MKI54_RS04095 (MKI54_000808)	851605..851865	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MKI54_RS04100 (MKI54_000809)	852206..852409	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MKI54_RS04105 (MKI54_000810)	852406..852642	-	237	WP_000349581.1	hypothetical protein	-
MKI54_RS04110 (MKI54_000811)	852867..853448	+	582	WP_001140693.1	histidine phosphatase family protein	-
MKI54_RS04115 (MKI54_000812)	853521..854138	-	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
MKI54_RS04120 (MKI54_000813)	854293..854787	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
MKI54_RS04125 (MKI54_000814)	855186..855608	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
MKI54_RS04130 (MKI54_000815)	855756..856472	+	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	817710..854787	37077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T236592 WP_000052076.1 NZ_CP092561:c851576-851388 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T236592 NZ_LR698987:1250684-1250863 [Leminorella richardii]
ATGGACAGCAAAAATGTAATAGCCATGATTGAGGCCGACGGATGGTATTTGGTTCGTATTAAAGGCAGTCATCATCAGTT
TAAGCATCCGCTAAAAAAGGGGCTGGTAACGGTAAAACATACACAAAAGGACATTCCGTTACCCACTCTAAAAAGTATTA
AAAGGCAGTCGGGAATTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT236592 WP_001059690.1 NZ_CP092561:c851865-851605 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT236592 NZ_LR698987:1250876-1251286 [Leminorella richardii]
ATGTTATACCCTGTCGCTATCGATCGCGGGGAAGAGTCTTTAGGGGTTCGAGTACCCGATATTCCCGGATGTTTTTCCGG
TGGGGATGACTATCAGGATGCACTGATAAGCGTAAAAGAGGCGATTGAGGCACATATCGAGCTATTGGTTGAAGATGGGG
ACGACGTACCGGCTGCAACCAACATTGAACGCTGGTTGAACGATCCTGAATATGCTGGTGCAATATGGGCGCTAGTCGAC
GTTGATATGGTTCGACTAATGGGAGGCGCTGAAAAAATTAACGTAACGTTACCCAAGCGACTGATCGCCAAGATAGACCG
TCTAGTTGCAACACGCCCTGAATTTAAAAGCCGCTCCGGCTTTCTGACGCAGGCTGCGCTAGAGCGAATTTCCATTGTGA
ATAACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56