Detailed information of TA system TA236591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 71270..71789
• Replicon: plasmid unnamed1
• Accession: NZ_CP092559
• Organism: Staphylococcus aureus strain VMRSA-WC052

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: MKI51_RS14450
• Protein ID: WP_000588503.1
• Coordinates: 71532..71789 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: MKI51_RS14445
• Protein ID: WP_000388479.1
• Coordinates: 71270..71539 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI51_RS14410 (MKI51_002860)	66841..67257	-	417	WP_001232859.1	hypothetical protein	-
MKI51_RS14415 (MKI51_002861)	67260..67544	-	285	WP_000353844.1	hypothetical protein	-
MKI51_RS14420 (MKI51_002862)	67910..68134	+	225	WP_000410576.1	hypothetical protein	-
MKI51_RS14425 (MKI51_002864)	69257..69937	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
MKI51_RS14430	70013..70579	+	567	Protein_80	hypothetical protein	-
MKI51_RS14435	70744..70944	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
MKI51_RS14440 (MKI51_002866)	70960..71139	+	180	WP_001120991.1	hypothetical protein	-
MKI51_RS14445 (MKI51_002867)	71270..71539	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKI51_RS14450 (MKI51_002868)	71532..71789	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
MKI51_RS14455 (MKI51_002869)	72426..73250	+	825	WP_001809248.1	hypothetical protein	-
MKI51_RS14460 (MKI51_002870)	73415..74029	-	615	WP_001261742.1	recombinase family protein	-
MKI51_RS14465 (MKI51_002871)	74479..75804	+	1326	WP_000969590.1	Y-family DNA polymerase	-
MKI51_RS14470 (MKI51_002872)	75797..76147	+	351	WP_000568378.1	hypothetical protein	-
MKI51_RS14475 (MKI51_002873)	76144..76323	+	180	WP_001021554.1	hypothetical protein	-
MKI51_RS14480 (MKI51_002874)	76459..76749	+	291	WP_000026576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / tet(S) / aac(6')-aph(2'') / VanHAX	-	1..78566	78566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T236591 WP_000588503.1 NZ_CP092559:71532-71789 [Staphylococcus aureus]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T236591 NZ_LR698987:1042298-1042585 [Leminorella richardii]
ATGAGTTATGCGGTCAAGTTTAGACGCGATGCATTGAAAGAGTGGCAAAAGCTGGATAAAGCCATTCAGCAGCAGTTTGC
TAAAAAGCTAAAGAAGTGCTGTGAAGAGCCTCATCTTCCTGCGGCTAGGCTAAGCGGCATGCCGGATTGCTATAAAATTA
AGCTTCGATCTTCTGGATTCCGCCTTGTTTATCAGGTTATTAATGACGAGCTGCTTGTTGTCGTCGTTGCAGTAGGCAAG
CGAGAGCGCAGCGGTGTTTATCATCTGGCTAGCGAGCGTATGAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT236591 WP_000388479.1 NZ_CP092559:71270-71539 [Staphylococcus aureus]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT236591 NZ_LR698987:1042050-1042301 [Leminorella richardii]
ATGCCAAATTTGATTTTAAGTGATATCAGCGCCAGCGTCAGTGAGTTAAAAAAGAACCCAATGGCAACGGTTAATGCAGG
AGCAGGATATCCAGTGGCAATTTTAAATCGTAACCAGCCCGCTTTTTACTGTGTCCCTGCGGAGCTGTATGAACAAATTT
TAGACGCGCTAGACGAGCATGAACTGGTGAAACTGGTCAAGGAGCGTCAGGGCCAGCCGCTGGTTGAGGTGGATTTGGAC
TCATATCTATGA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7