Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2120088..2120602 Replicon chromosome
Accession NZ_CP092558
Organism Staphylococcus aureus strain VMRSA-WC052

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI51_RS10525 Protein ID WP_000581792.1
Coordinates 2120088..2120297 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI51_RS10530 Protein ID WP_000138932.1
Coordinates 2120309..2120602 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI51_RS10505 (MKI51_002080) 2115596..2116954 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI51_RS10510 (MKI51_002081) 2116969..2118039 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI51_RS10515 (MKI51_002082) 2118357..2119559 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI51_RS10520 (MKI51_002083) 2119823..2119960 - 138 WP_000828354.1 Lmo0850 family protein -
MKI51_RS10525 (MKI51_002084) 2120088..2120297 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI51_RS10530 (MKI51_002085) 2120309..2120602 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI51_RS10535 (MKI51_002086) 2120768..2122261 + 1494 WP_000571554.1 cardiolipin synthase -
MKI51_RS10540 (MKI51_002087) 2122289..2122936 + 648 WP_001187633.1 HD domain-containing protein -
MKI51_RS10545 (MKI51_002088) 2123849..2124721 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI51_RS10550 (MKI51_002089) 2124807..2125448 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236586 WP_000581792.1 NZ_CP092558:c2120297-2120088 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236586 NZ_LR698982:3008276-3008569 [Gordonibacter urolithinfaciens]
ATGTATGCAGTGAAGTTCACGAAGCAGGCTGCAAAGGATGCAAAGAAGCTTAAAGCCGCTGGTCTCGACAAGAAGGCGAA
AGCACTTGTCGAGGTGCTTAAGAATGATCCCTTTCAAGAGGCTCCTGCCTACGAAACGCTTGTGGGGAACCTCTCGGGCC
TCTATTCGCGGCGCATCAACCTGCAGCATCGGCTCGTTTATCAGGTATATGCAGAGCCCTTTGCCGAGGGTGGCGAGGAA
TTCGATGGCACGGTCAAAGTCGTGCGCATGTGGACCCATTACGAGGGACTCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236586 WP_000138932.1 NZ_CP092558:c2120602-2120309 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236586 NZ_LR698982:3008022-3008273 [Gordonibacter urolithinfaciens]
ATGACTTCGATCACTGCAACGGCTTTGCGGCAGAATATCTACCAGACCATCGCCCAGGTGAACGAGAACTGCACGCCCAT
CGCCATCACGAACAGCAAGGGGAAGGGGGCAGTGCTTATCGGCGAATCCGATTGGGCGGCTATCGAGGAAACGCTTTTCC
TCAACGGCATTCCCGGCATGAGCGAATCGCTGCGGGCCGGCATGGCGGAAAAACCCGAGGACTGCCTTTCTGAAGACCGA
CTTGAGTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References