Detailed information of TA system TA236585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2098829..2099358
• Replicon: chromosome
• Accession: NZ_CP092558
• Organism: Staphylococcus aureus strain VMRSA-WC052

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKI51_RS10425
• Protein ID: WP_000621175.1
• Coordinates: 2098829..2099191 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MKI51_RS10430
• Protein ID: WP_000948331.1
• Coordinates: 2099188..2099358 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI51_RS10405 (MKI51_002061)	2095807..2096577	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
MKI51_RS10410 (MKI51_002062)	2096552..2097031	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MKI51_RS10415 (MKI51_002063)	2097033..2097359	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MKI51_RS10420 (MKI51_002064)	2097478..2098479	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MKI51_RS10425 (MKI51_002065)	2098829..2099191	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKI51_RS10430 (MKI51_002066)	2099188..2099358	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MKI51_RS10435 (MKI51_002067)	2099443..2100591	-	1149	WP_001281154.1	alanine racemase	-
MKI51_RS10440 (MKI51_002068)	2100657..2101016	-	360	WP_000581197.1	holo-ACP synthase	-
MKI51_RS10445 (MKI51_002069)	2101020..2101511	-	492	WP_001205912.1	PH domain-containing protein	-
MKI51_RS10450 (MKI51_002070)	2101498..2103081	-	1584	WP_001294626.1	PH domain-containing protein	-
MKI51_RS10455 (MKI51_002071)	2103074..2103553	-	480	WP_001287087.1	hypothetical protein	-
MKI51_RS10460 (MKI51_002072)	2103761..2104321	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T236585 WP_000621175.1 NZ_CP092558:c2099191-2098829 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T236585 NZ_LR698982:2211262-2211627 [Gordonibacter urolithinfaciens]
ATGTACAAAGTAGAGTTGTACCATGATGCAAAGGGGCGATCGGAGCTGCTCGACACTTTGCGCAAGCTGGAGGATAAGAG
CAGTACGGACAAAGAGGCGAGGACGGCGTATTTAAGCATGCTCAAGGCTATTTCCGAGCTGGAAGGGCATGGCACGAGGA
TCGGTATGCCGACCGTTCGCCATGTGAGAGGCGAGATATGGGAGCTCAGGCCGAAATCCCAGCGGGTGTTCTTTTTCTTC
TGGAAGGACAATACGTTCGTTTTGCTGCATTCCTATATTAAGAAAACCCAGAAGACGCCTCGGCGCGAGATAATGAAAGC
CGAGCGTAAAAAACGCGATTGGATAGCGAGGCATGCCGATGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT236585 WP_000948331.1 NZ_CP092558:c2099358-2099188 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT236585 NZ_LR698982:2211620-2211919 [Gordonibacter urolithinfaciens]
ATGAGTAAAAACGAGGGTTGCTATACCTTCCAAGACTACCTGAAAGAAGCGAAGCTCACTCCTGCCGAGCGCGAGGAGAT
CCGGCTGGACGTGGAGCTCACGGGGAGGCTGATCGAGGCGCGCGAGGCGCGGGGGATGACGCAGCGCGATTTGGCGGAGG
CGAGCGGCCTCAAGCAGCCGGCCATTGCCCGCATCGAGTCGCATCGCTCGGTGCCGCGCGTGGACACGCTGCTCAAGCTG
CTGGTGCCGCTGGGCTACACGCTCAAGATCGTGCCGCTCGAAGGCAGCGACCGTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1