Detailed information of TA system TA236583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1885913..1886689
• Replicon: chromosome
• Accession: NZ_CP092558
• Organism: Staphylococcus aureus strain VMRSA-WC052

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: MKI51_RS09065
• Protein ID: WP_000031108.1
• Coordinates: 1885913..1886065 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: MKI51_RS09070
• Protein ID: WP_001251224.1
• Coordinates: 1886090..1886689 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKI51_RS09050 (MKI51_001793)	1882115..1882936	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
MKI51_RS09055 (MKI51_001794)	1883389..1884774	-	1386	WP_000116228.1	class II fumarate hydratase	-
MKI51_RS09060 (MKI51_001795)	1884970..1885365	-	396	WP_000901023.1	hypothetical protein	-
MKI51_RS09065 (MKI51_001796)	1885913..1886065	-	153	WP_000031108.1	SAS053 family protein	Toxin
MKI51_RS09070 (MKI51_001797)	1886090..1886689	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
MKI51_RS09075 (MKI51_001798)	1886848..1887318	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
MKI51_RS09080 (MKI51_001799)	1887323..1888450	-	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
MKI51_RS09085 (MKI51_001800)	1888601..1889323	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
MKI51_RS09090 (MKI51_001801)	1889316..1890773	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T236583 WP_000031108.1 NZ_CP092558:c1886065-1885913 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T236583 NZ_LR698979:565808-566077 [Bifidobacterium thermophilum]
TTGGCTTGGAAGATTGAAATCGATAAGGGCGTCCAGCGTTCGATGAAGAAACTGGATCGCCATGTCGCCAAGAGAATCAT
CGCAAAACTTAGGGAAATTTCACAGTTGGAAGATCCAAGAAGCACTGGCAAAGCTCTTGTCGGTAATCTGGCAGGGCTGT
GGCGTTACCGTGTTGGGGACTATCAGATCGTGTGCGATATAGAAGATGAGGTTCTACTGATTCTTGTGGTTGATGTCGCG
CACAGGAGCAAGGTGTACAAGCGCCGTTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT236583 WP_001251224.1 NZ_CP092558:c1886689-1886090 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT236583 NZ_LR698979:565588-565824 [Bifidobacterium thermophilum]
ATGGCCACGGCTACTGCAGCATTGAGGATGCCTGTCGAATTGGCGGCTCGTTACGATCGTTTGGCGAAAGCAACTGGCCG
TACGAAGACGTTTTATATGAACGAAGCCCTTACCGAGTCAATCGATAGGCTTGAGTATGAATACGGCATTATGAAAAAGG
TTGAGGATTGGCGTGCCGGCATACTCGAGACTGTGACGCTCGACGAGCTGGAGGAAAGCCTTGGCTTGGAAGATTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4