Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2124596..2125110 Replicon chromosome
Accession NZ_CP092556
Organism Staphylococcus aureus strain VMRSA-WC062

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MKI64_RS10545 Protein ID WP_000581792.1
Coordinates 2124596..2124805 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag MKI64_RS10550 Protein ID WP_000138932.1
Coordinates 2124817..2125110 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKI64_RS10525 (MKI64_002086) 2120104..2121462 - 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKI64_RS10530 (MKI64_002087) 2121477..2122547 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MKI64_RS10535 (MKI64_002088) 2122865..2124067 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MKI64_RS10540 (MKI64_002089) 2124331..2124468 - 138 WP_000828354.1 Lmo0850 family protein -
MKI64_RS10545 (MKI64_002090) 2124596..2124805 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MKI64_RS10550 (MKI64_002091) 2124817..2125110 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
MKI64_RS10555 (MKI64_002092) 2125276..2126769 + 1494 WP_000571554.1 cardiolipin synthase -
MKI64_RS10560 (MKI64_002093) 2126797..2127444 + 648 WP_001187633.1 HD domain-containing protein -
MKI64_RS10565 (MKI64_002094) 2128357..2129229 - 873 WP_000725802.1 membrane protein insertase YidC -
MKI64_RS10570 (MKI64_002095) 2129315..2129956 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T236574 WP_000581792.1 NZ_CP092556:c2124805-2124596 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T236574 NZ_LR698955:c2226283-2226023 [Fusobacterium nucleatum]
ATGAATAATTTAGTATGGACACATAAAGCTTGGCAAGATTATCTATATTGGCAAACTCAGGATAAAAAAACTTTAAAAAA
GATAAATGAATTAGTCAAAGATATTGAAAGAAATGGAGCTTTAAATGGGATAGGCAAGCCAGAAGTATTAAAAAATGAAA
ATGCATATAGTAGAAGAATTGATGAAAAAAATAGACTGGTTTATAGAATTGTAGATGGGGTTATATGGATAATTGCTTGT
AAAGGTCATTATGAGGAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT236574 WP_000138932.1 NZ_CP092556:c2125110-2124817 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT236574 NZ_LR698955:c2226530-2226276 [Fusobacterium nucleatum]
ATGTTAGCAATTAATTATACAACTTTGAGAACCAATTTAAAAAGCTATTTTGATAAAGCAGTAGATAATGATGAAGATAT
TATTATAACAAGAAAAAATGAAAGAAATGTTGTTCTATTAAGTCTTGATAAATATAATGAGTTTTTAAAAGCTATGAGAA
ATCTTGAATATATGACAAAAATAAGAGAAGGAATAGCTGAATTAGAGGCAGGAAAAGGAGAAATCCATGATTTAATAGAG
GTTGATGATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References